Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by...

Although winter dysentery (WD) has been suspected to occur frequently in Korea, to date the exact epidemiology of WD has remained unknown. Therefore, we investigated the causative agents of WD by using electron microscopy, ELISA, RT-PCR, and nested PCR from 97 fecal samples of 32 WD-affected herds collected from 8 provinces during 2002-2004. The bovine coronavirus (BoCV) was consistently detect...

Foams of bovine serum albumin (BSA) have been utilized for synthesizing in situ protein capped Ag-Pt alloy nanoparticles. The in vitro cytotoxicity and the rate of proliferation of human gingival fibroblasts (HGFs) in presence of the above synthesized alloy nanoparticles is investigated. Expression profile of protein involved in detoxification, i.e. metallotheonein (MT) were assayed by ELISA an...

A total of 119 unpasteurized milk samples taken from 63 cows, 29 goats and 27 sheep bred on 8 farms situated on the territory of the Lublin province (eastern Poland), an area of risk of tick-borne encephalitis (TBE), were examined for the presence of RNA of tick-borne encephalitis virus (TBEV) by the nested RT-PCR method. Milk samples were also tested for the presence of anti-TBEV antibodies by...

an outbreak of a lethal disease was reported in 4–6-month-old holstein calves in a feedlot around tehran. the signs of central nervous system and gastrointestinal system (gi) involvement were observed in the diseased animals. necropsy samples of gi, liver, kidney, spleen and lung from 3 died animals were prepared for histopathological examination. blood and formalin-fixed ear notch samples of 6...

tomato is one of the most important vegetables in iran and throughout the world with crop loss caused by several viruses. this survey was carried out to detect the viruses causing mosaic symptoms on tomato in yazd city (iran). a total of 451 samples of tomato leaves showing mosaic symptoms were collected from fields and greenhouses during 2012-2013 growing season. collected samples were analyze...

towelve calves of 4-10 months old with clinical signs suspected to bovine viral diarrhea virus (bvdv) infection were selected for this study. histopathologic sections were performed on formalin fixed paraffin-embded ear notch biopsies, mounted on poly-l-lysine coated slides and stained for bvdv by anti-bvdv monoclonal antibody labelled with fluorescein isothiocyanate. stained sections were exam...

The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method wi...

This paper presents the ELISA speaker segmentation approach applied on multiple audio channel meeting recordings in the framework of NIST RT’04s meeting (spring) evaluation campaign. As done for BN data speaker segmentation, the ELISA “meeting” system involves two speaker segmentation systems developed individually by the CLIPS and LIA laboratories. The main originality consists in a “two-axis”...