The present investigation was conducted to develop a protocol for rapid plant regeneration in potato. The newly emerge sprout of six potato cultivars were used as explant. Varietal response on in vitro regeneration under different hormonal concentration and combination were studied. MS medium supplemented with 0.5, 1.0, 1.5, 2.0, 4.0 mg L of KIN and IAA in combination of both were employed for ...

who recommends artemisinin-based combination therapies (acts) as the most effective choice to treat malaria. for developing transgenic plants with high accumulation of artemisinin (by introducing genes encoding enzymes which regulate the biosynthetic pathway of artemisinin), an efficient protocol for tissue culture and plant regeneration is necessary. in the present study, leaf explants of arte...

An effi cient and reproducible plant regeneration procedure is essential for introducing genes of interest in important crops through genetic transformation. However, some crops, such as soybean [Glycine max (L.) Merrill], are diffi cult to manipulate in vitro, often depending on their genotype, and the reproduction of the established protocols is not always possible. The purpose of this paper ...

saffron (crocus sativus l.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of cellulase, pectinase and hemicellulase and embedded in ca-alginate beads. they were cultured with or without nurse cells in ms medium supplemented with 2,4-d and 6-benzylaminopurine at 25°c. after several changes of medium, cell-clusters appeared on the surface of the ca-algi...

An efficient protocol of common bean transformation using Agrobacterium tumefaciens is still unavailable. Several regeneration reports are available using cotyledonary node or embryonic axis as the starting explants. These explants were used for direct organogenesis exploiting the meristems for direct shoot production. Attempts were made to transform cotyledonary node explants and embryonic axe...

Plants can be regenerated from various explants/tissues via de novo shoot meristem formation. Most of these regeneration pathways are indirect and involve callus Besides plant hormones, the role polyamines (PAs) has been implicated in processes. Interestingly, lateral root primordia (LRPs) Arabidopsis directly converted to meristems by exogenous cytokinin application. In this system, no formati...

A rapid and efficient protocol was developed for inducing direct organogenesis using shoot tip explants of Vernonia cinerea L. Shoot tip explants were cultured on MS medium supplemented with different concentrations of BAP (2.22 to 22.20 μM/l) and KN (2.42 to 23.20 μM/l) for direct shoot induction. The frequency of multiple shoot induction and proliferation increased with increasing concentrati...

An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The resu...

The features of creating decorative forms from Scots pine trees are considered. It has been established that, as a result mechanical damage (death an apical bud or shoot) and subsequent regeneration processes in trunks, two main directions post-traumatic deformations young trunks stand out - violation monopodial branching occurrence multi-stemming. In the case death shoot, early spring, growth ...

The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA...