Abstract Marine Vibrio species are natural degraders of chitin and usually secrete high levels chitinolytic enzymes to digest recalcitrant chitooligosaccharides. This study used an endochitinase ( Vh ChiA) from campbellii produce high-quality chitobiose crustacean chitins. The enzyme was shown be fully active stable over 24 h when BSA as additive. When different sources were tested, ChiA prefer...

Point-of-care testing (POCT) devices are becoming increasingly popular in the medical community as an alternative to conventional laboratory testing, especially for home treatments or other forms of outpatient care. Multiple-use chemical sensors with minimal requirements disposables among most practical and cost-effective POC diagnostic instruments, managing chronic conditions. An affordable, s...

In this study, optimization of Qeshm power and water desalting cogeneration plant has been investigated. The objective functions are related to maximizing exergetic efficiency and minimization of exergoeconomic and exergoenvironmental parameters. Also, the integration of RO desalination with the existing plant has been evaluated based on these analyses. This plant includes two MAPNA 25 MW gas t...

Dinitrophenylation-The amino-terminal residue was determined on. a sample of the peptide before desalting. Approximately 1 pmole of the peptide was submitted to dinitrophenylation for 6 hours with the reaction mixture maintained at pH 8.5 with the aid of a pH stat. The solution was evaporated to dryness and triturated with dry ethyl acetate. Estraction was continued until all the yellow color w...

A new MALDI-TOF based mini-sequencing assay termed VSET was developed for genotyping of SNPs. In this assay, specific fragments of genomic DNA containing the SNP site(s) are first amplified, followed by mini-sequencing in the presence of three ddNTPs and the fourth nucleotide in the deoxy form. In this way, the primer is extended by only one base from one allele, while it is typically extended ...

The maximum achievable tyrosination level of neurotubulin, in vitro, is about 50%. We have developed a method to obtain a complete separation of the tyrosinatable and nontyrosinatable species. We use an immunoaffinity column, with coupled YL 1/2 monoclonal antibody (anti-Tyr-tubulin) and rapid desalting methods. Both subpopulations can be obtained in a polymerizable, apparently native, form. We...

ments. Blood Coagul Fibrinolysis 1999;10:285–9. 10. Morris A, Marsh JJ, Burrows CM, Chiles PG, Konopka RG, Pedersen CA. Urine and plasma levels of fibrinopeptide B in patients with deep vein thrombosis and pulmonary embolism. Thromb Res 2003;110:159–65. 11. De Palo EF, Gatti R, Lancerin F, De Palo CB, Cappellin E, Solda G, et al. Effects of acute, heavy-resistance exercise on urinary peptide ho...