While the postulates of Koch have never been fulfilled in granuloma inguinale, there is much presumptive evidence to indicate that Donovania granulomatis is the aetiological agent of the disease. The prototype of D. granulomatis was isolated originally by Anderson (1943), and since that time at least six other investigators (Beveridge, 1945; Dienst, 1948; Goldberg, Weaver, and Packer, 1953; Jen...

The measurement of both immune complex-bound and free unbound tumor-associated antigen was evaluated independently on a panel of sera from colon cancer patients by radioimmunoassay (RIA). A monoclonal antibody (mAb 46.3) raised against secreted antigens from human colon cancer cells in vitro was utilized in the RIA. When circulating immune complexes alone were analyzed, the data demonstrated th...

While the postulates of Koch have never been fulfilled in granuloma inguinale, there is much presumptive evidence to indicate that Donovania granulomatis is the aetiological agent of the disease. The prototype of D. granulomatis was isolated originally by Anderson (1943), and since that time at least six other investigators (Beveridge, 1945; Dienst, 1948; Goldberg, Weaver, and Packer, 1953; Jen...

Treatment of lambs (initial BW 28 kg) for 24 d with a combined implant containing 40 mg of trenbolone acetate (TBA) and 8 mg of estradiol (E2) increased ADG 25% ( P < .05, n = 8) and feed efficiency 23% ( P < .05, n = 2) compared with unimplanted lambs. By d 3 following implantation, sera from wethers implanted with TBA + E2 showed 32% (307 vs 233 ng/mL) increases ( P < .001, n = 8) in IGF-I co...

Philander frenata and Didelphis marsupialis harbor parasitism by Trypanosoma cruzi without developing any apparent disease and on the contrary to D. marsupialis, P. frenata maintains parasitism by T. cruzi II subpopulations. Here we compared the humoral immune response of the two didelphids naturally and experimentally infected with T. cruzi II group, employing SDS-PAGE/Western blot techniques ...

Antibody avidity, the strength of the multivalent interaction between antibodies and their antigens, is an important characteristic of protective immune responses. We have developed an inhibition enzyme-linked immunosorbent assay (ELISA) to measure antibody avidity for the capsular polysaccharide (PS) of Neisseria meningitidis group C (MnC) and determined the avidity constants (K(D)s) for 100 s...

The teichoic acid of streptococcal Group N, with end groups of galactose phosphate, had been shown to cross-react with antipneumococcal sera of types VI, XIV, XVI, and XXVII. End groups of D-galactose-1-phosphate in the phosphogalactans of Sporobolomyces yeasts made it predictable that these galactans would precipitate the same antipneumococcal sera and also antisera to streptococcal Group N. T...

Rabbi ts immunized with purin-6-oyl-protein conjugates form antibodies with purine specificity. These antibodies are capable of fixing complement (C t) in the presence of thermally denatured deoxyribonucleic acid (DNA). This reaction was found to be specifically inhibited by purines and purine derivatives (1). Since the purines in D N A are linked to the deoxyribose-phosphate "backbone" a t the...

BACKGROUND The dust mite Lepidoglyphus destructor is a major cause of allergic diseases among farmers. We have previously cloned and sequenced two isoforms of the major allergen Lep d 2 (formerly designated Lep d 1) and found significant homology to group 2 allergens of the house dust mite species Dermatophagoides. We now report on the production and characterization of recombinant Lep d 2. O...

Healthy human sera (HHS) contain naturally acquired enterococcal antibodies which promote neutrophil-mediated killing. The target antigens remain unknown. The present study used a capsular polysaccharide (CPS)-enzyme-linked immunosorbent assay (ELISA) to investigate whether the HHS antibodies of 12 healthy donors bound to the CPS of four E. faecalis serotypes (CPS-A to CPS-D) and then employed ...