Background. Cryopreservation of spermatozoa with the best morphofunctional characteristics may allow their use for assisted reproductive technologies in goat breeding throughout year to effectively increase livestock. Aim. To evaluate influence seasons on morphological and morphometric fresh cryopreserved sperm. Methods. The ejaculate samples three sexually mature bucks Saanen breed were obtain...

The freezing and storage of the sperm are used cryopreservation germplasm in livestock breeding, genetic improvement indigenous species, preservation rare races, successful tolerance to environmental changes international exchanges. Both thawing process causes large volume cell fluid. Spermatozoon removes most its cytoplasm at differentiation stages lacks cytoplasmic component that contains ant...

Cryopreservation of growing oocytes enriches the choice of timing and location of artificial embryo production. However, completion of oocyte growth after warming is crucial when using such cryopreserved oocytes. Our research objective was to develop a sequential system that incorporates cryopreservation of growing bovine oocytes and their subsequent in vitro growth. Oocyte-granulosa cell compl...

Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopre...

because of the importance of iranian rice germplasm and its application in the rice breeding programs and international genetic resources exchanges, some researches are necessary to preserve these valuable genetic resources. for this purpose, the cryopreservation of the biological samples in liquid nitrogen at -196 °c can be used. in this research, the cryopreservation of the zygotic embryos of...

The preservation of the female portion of livestock genetics has become an international priority; however, in situ conservation strategies are extremely expensive. Therefore, efforts are increasingly focusing on the development of a reliable cryopreservation method for oocytes, in order to establish ova banks. Slow freezing, a common method for cryopreservation of oocytes, causes osmotic shock...

OPINION STATEMENT With improving survival rates for pediatric and young adult cancer patients, considerations regarding the long-term effects of therapy have become more important. Cancer therapies are known to pose reproductive risks, though the effects may be unpredictable. All at-risk patients should have a discussion about potential treatment-related infertility before the onset of cancer t...

Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed chromosomal stability through karyotype analysis, determined whe...

INTRODUCTION Several options are available for preserving fertility before cytotoxic treatment, including ovarian tissue cryopreservation. Most reported surgical techniques include electrocoagulation. Our hypothesis is that avoidance of electrocoagulation may decrease ovarian cortex injury during cryopreservation procedures. CASE PRESENTATION We report a laparoscopic technique of whole-ovary ...

OBJECTIVES Human mesenchymal stem cells (MSCs) are efficacious in various cellular therapeutic applications and have been isolated from several tissues. Recent studies have reported that human tonsil tissue contains a new source of progenitor cells, potentially applicable for cell-based therapies. Information about the effects of donor age, long-term passage and cryopreservation are essential f...