Background: Umbilical cord blood hematopoietic stem cells (UCB-HSCs) are an attractive source for transplantation. The generation of megakaryocyte-committed cells could lead to shorten period of thrombocytopenia after HSCs transplantation. Platelet lysate (PL) unlike fetal bovine serum (FBS) can prevent immune problems as well as avert transmission of certain diseases to the recipient. In this ...

backgroundresistin is reported as an antagonistic hormone of insulin and in the literature. nowadays, importance of resistin is being more highlighted in the metabolism of mother and fetus during pregnancy. this study aimed to assess the level of resistin in the umbilical cord and blood sugar at birth and evaluate the association of resistin and neonatal birth weight.materials and methodsthis c...

background: abnormal umbilical cord ph can be indicated a distress imposed of newborn and can also be useful in determining the prognosis of the newborn. there are different views about the effect of the neonatal delivery method on the blood gas analysis of the umbilical artery. this study aimed to determine the effect of the method of delivery on umbilical artery ph. methods: in this cross –se...

how to cite this article: mirzarahimi m, ahadi a, bohlooli sh, namakikhalajan e, barak m. antioxidant levels in cord blood of term neonates and its association with birth weight. iran j child neurol. winter 2016; 10(1):31-34.   abstract objective due to excessive production of free radicals and antioxidants evolved mechanisms against oxidative stress, infants are very vulnerable. as there was a...

a quasi experimental study was conducted in the labour rooms of one of the hospital's of the university of   medical science of iran, to compare the effect ofcord blood drainage and oxytocin injection into the umblicat   vein in the duration of the third stage of labour.   a total of 180 women in the course of normal pregnancies with no complications and normal vaginal   dd ivery were sele...

We have previously found that the synthetic polyamine tetraethylenepentamine (TEPA) significantly delayed differentiation and prolonged expansion of cord-blood derived HPC in cytokine-supplemented cultures. Most HPC have the CD34+CD38+ phenotype, but the minority CD34+38- cells are primitive subset of HPC that have the potential for long-term repopulation in vivo. We investigated the effect of ...

Ex vivo expansion of cord blood cells generally results in reduced stem cell activity in vivo. Glycogen synthase kinase-3beta (GSK-3beta) regulates the degradation of beta-catenin, a critical regulator of hematopoietic stem cells (HSCs). Here we show that GSK-3beta inhibition activates beta-catenin in cord blood CD34(+) cells and upregulates beta-catenin transcriptional targets c-myc and HoxB4,...

The optimisation of haematopoietic stem and progenitor cell expansion is on demand in modern cell therapy. In this work, haematopoietic stem/progenitor cells (HSPCs) have been selected from unmanipulated cord blood mononuclear cells (cbMNCs) due to adhesion to human adipose-tissue derived stromal cells (ASCs) under standard (20%) and tissue-related (5%) oxygen. ASCs efficiently maintained viabi...

Ex vivo expansion of haematopoetic cells by application of specific cytokines is one approach to overcome boundaries in cord blood transplantation due to limited numbers of haematopoetic stem cells. While many protocols describe an effective increase of total cell numbers and the amount of CD34-positive cells, it still remains unclear if and how the procedure actually affects the cells' propert...

UNLABELLED In this study, carboxylic acid functionalized single walled carbon nanotubes (f-SWCNT-COOH) was shown to support the viability and ex vivo expansion of freeze-thawed, non-enriched hematopoietic stem and progenitor cells (HSPC) in human umbilical cord blood-mononucleated cells (UCB-MNC). Our in vitro experiments showed that f-SWCNT-COOH increased the viability of the CD45(+) cells eve...