Serum cholinesterase activity and the dibucaine numbers have been determined by using a hydrogen peroxide electrode and the enzyme choline oxidase immobilized on a nylon net. The analysis procedure is extremely simple and very fast allowing 30 cholinesterase determinations per hour. Both cholinesterase activity and dibucaine number measurements could be performed in 5 min and by using serum sam...

Sera of various phenotypes at serum cholinesterase locus 1, including the newly recognized phenotypes E1 aE1j, E1 uE1j, and E1 fE1J, were studied by immunodiffusion and rocket immunoelectrophoresis. The sera containing the E1j allele show reduced numbers of immunologically active cholinesterase molecules. This finding is consistent with the previously advanced hypothesis that E1j results in red...

The influence of ketamine upon human plasma cholinesterase (E.C. 3.1.1.8) was determined by means of a colorimetric assay technique at 25 degrees C, pH 7.7 with butyrylthiocholine as substrate. Ketamine caused a reversible inhibition of cholinesterase (pI50= 2.75; apparent Ki=4.94X10(-4) M). The kinetic analysis of the inhibitory action revealed a mixed competitive/non-competitive type of mecha...

The aim of this study was to determine whether there was any relationship between body fat and pseudo(or plasma) cholinesterase. Hutchinson, McCance & Widdowson (I~sI), in reporting their studies of undernutrition and feeding, pointed out that, at the end of rehabilitation when men became ‘fat’, the average pseudo-cholinesterase was higher than normal. It therefore seemed of interest to investi...

Plasma cholinesterase (PCHE) activity was determined using a colorimetric method in 11 healthy non-pregnant women, 11 healthy pregnant women at term, and 11 pre-eclamptic pregnant women at term. The mean plasma cholinesterase activities for healthy non-pregnant women, healthy pregnant women and pre-eclamptic pregnant women were 438 +/- 81,257 +/- 25 and 173 +/- 18 units . ml-1, respectively. Ou...

伴 隆志 ・永田 親 義 The inhibition of cholinesterase by some of carbamates has attracted the attention of many researchers in relation to the clinical usefulness (1) and insecticidal activity (2, 3) of the compounds. In this paper the author's chief concern is mainly devoted to investigating the rela tionship between the electronic structure of several structurally related carbamate type cholinesteras...

OBJECTIVE To review the literature relating to the use of acetyl cholinesterase inhibitors in Parkinson's disease dementia (PDD). METHOD MEDLINE (1966--December 2004), PsychINFO (1972--December 2004), EMBASE (1980--December 2004), CINHAL (1982--December 2004), and the Cochrane Collaboration were searched in December 2004. RESULTS Three controlled trials and seven open studies were identifie...

This report assesses the value of cholinesterase (ChE) activity as an indicator of liver function and analyses its usefulness in the diagnosis of jaundice. A rapid method of determination has been used, and alterations in ChE activity in conditions other than liver disease have been studied to permit a fuller and more accurate survey of its value as a liver function test. The level of serum cho...

The kinetic characteristic (Km) of cholinesterase from the crab Chasmagnathus granulata, the shrimp Farfantepenaeus paulensis and the fish Odontesthes bonaeriensis were compared and correlated with the anticholinesterasic effect of eserine (physostigmine). For the crustaceans, the estimated Km values were about 5-8 times higher than that estimated for the fish (0.04 mM). In the crab and the shr...