In the present study, stigmasterol has been reported from Pedalium murex L. of pedaliaceae family in vivo and in vitro. Quantification data revealed that the total sterol content was higher in callus than leaves. The in vitro studies showed that the maxmium amount of total sterol was found in 6 weeks old tissue and minimum in 2 weeks old callus culture. In vivo studies showed that higher conten...

In order to investigate the genetic variation in tissue culture response and to ®nd the cultivars with high regeneration ability for genetic transformation, twenty®ve turf-type tall fescue (Festuca arundinacea Schreb.) cultivars, including many elite ones released recently, were evaluated for their callus induction and plant regeneration responses. Callus induction was initiated from mature see...

Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together wi...

In this study, callus of P. tunicoides was used to induce adventitious roots. It was found that the medium with indole-3-butyric acid (IBA) was most suitable for induction of P. tunicoides adventitious roots from callus. To investigate the effects of concentration and type of auxins on adventitious roots induction, an orthogonal test was deployed. It was shown that 2,4Dichlorophenoxyacetic acid...

turmeric (cucurma longa) has been used as an antioxidant agent in medical as well as health sciences some years ago. however, it has not been applied in tissue culture till now. browning tissues is result of tissue damages caused by polyphenoloxidase enzyme. in order to study this problem and reduce browning, explants are cultured in culture media containing antioxidants including citric acid, ...

Embryogenic callus culture of carrot (Daucus carota L.) was induced from the hypocotyl explants in MS medium supplemented with Casein hydrolysate, 2,4-D and BAP for somatic embryogenesis In vitro cell suspension cultures for high multiplication rate. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium The mature somatic embryos were encapsulated in a Sodium alginate mi...

Partially expanded male catkins of swamp white oak (Quercus bicolor Willd.) and red oak (Quercus rubra L.) were cultured on Murashige and Skoog (MS) medium supplemented with BA or 2,4-D. Explants on 2,4-D produced a yellow embryogenic callus originating from the junction of the pedicel and peduncle. Subsequent transfers to MS with BA and then MS without growth regulators resulted in callus prol...

Ascochyta blight caused by Ascochyta rabiei (Pass.) Labrousse is a major biotic constraint in production of chickpea. In the present investigation, all chickpea genotypes [E100Y (m), Gaurav, Pb-7 and L550] induced 100% callus on standard medium with greenish colour and fragile structure. These calli were used for in vitro screening against pathogen, A. rabiei culture filtrate at 0, 5, 10, 15 an...

Toddalia asiatica(Rutaceae) is a woody liana used traditionally in the treatment of malaria, sprains, cough, fever, neuralgia, epilepsy, dyspepsia and other disease conditions. The extracts of the plant have been reported to have anticancer and anti-HIV activities. These activities are mainly attributed to benzophenanthridine alkaloid i.e., nitidine. The yield of the nitidine in intact plant is...

Differentiation and morphogenetic potential of Allium commutatum Guss. callus tissue was investigated in five subcultures. Callus tissue was derived from root tips of in vitro grown seedlings and maintained on MS medium supplemented with 3% sucrose, 0.8% agar, 4.5 pM 2,4-D and 4.6 pM kinetin. Pieces of calli were subcultured on media with different concentrations and combinations of 2,4-D (1.0,...