We have shown previously that the Ca channel b3 subunit is capable of modulating tonic G-protein inhibition of a1A and a1B Ca channels expressed in oocytes. Here we determine the modulatory effect of the Ca channel b3 subunit on M2 muscarinic receptor-activated G-protein inhibition and whether the b3 subunit modulates the G-protein sensitivity of a1A and a1B currents equivalently. To compare th...

BACKGROUND Smoking increases CD risk. The aim was to determine if smoking cessation at, prior to, or following, CD diagnosis affects medication use, disease phenotypic progression and/or surgery. METHODS Data on CD patients with disease for ≥5 yrs were collected retrospectively including the Montreal classification, smoking history, CD-related abdominal surgeries, family history, medication u...

Biodegradation, Biotransformation, and Biocatalysis (B3) R. E. Parales,* N. C. Bruce, A. Schmid, and L. P. Wackett Department of Microbiology and Center for Biocatalysis and Bioprocessing, The University of Iowa, Iowa City, Iowa; Department of Biochemistry, Molecular Biology and Biophysics, Biotechnology Institute, University of Minnesota, St. Paul, Minnesota; Institute of Biotechnology, Univer...

Metastatic breast cancer is the second leading cause of cancerrelated deaths among women. Triple-negative breast cancer (TNBC) is a highly aggressive subcategory of breast cancer and currently lacks well-defined molecular targets for effective targeted therapies. Disease relapse, metastasis, and drug resistance render standard chemotherapy ineffective in the treatment of TNBC. Because previous ...

Our objective was to isolate a prelysosomal compartment involved in receptor-mediated endocytosis in human epidermoid carcinoma (A431) cells. The isolation protocol involves density modification of endosome elements in A431 cells, caused by the receptor-dependent binding and internalization at 20 degrees C of colloidal gold-transferrin receptor antibody (B3/25) particles. The use of 125I-labell...

viral protein-1 (vp1) is a major capsid protein of coxsakievirus b3 (cvb3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems vp1 can be considered as a vaccine candidate against cvb3 infection. in this study, cdna of vp1 was prepared, cloned into pet expression vector and...

287 Abstract Introduction: Peri-prosthetic fracture is the third cause in frequency in hip revision following aseptic loosening and infection. The ones that show associated with a loosened stem (B2) or a bone stock deficit (B3) should receive femoral revision. Materials and Methods: We studied retrospectively 38 patients with types B2 and B3 femur peri-prosthetic fracture treated with distal fi...

In this paper we investigate the decidability and complexity of problems related to braid composition. While all known problems for a class of braids with three strands, B3, have polynomial time solutions we prove that a very natural question for braid composition, the membership problem, is NP-complete for braids with only three strands. The membership problem is decidable in NP for B3, but it...

The aim of this paper is a reduction algorithm for a basis b1, b2, b3 of a 3-dimensional lattice in R n for fixed n ≥ 3. We give a definition of the reduced basis which is equivalent to that of the Minkowski reduced basis of a 3-dimensional lattice. We prove that for b1, b2, b3 ∈ Z, n ≥ 3 and |b1|, |b2|, |b3| ≤ M , our algorithm takes O(log M) binary operations, without using fast integer arith...

The nuclear lamina (NL) is a filamentous protein meshwork, composed essentially of lamins, situated between the inner nuclear membrane and the chromatin. There is mounting evidence that the NL plays a role in spermatid differentiation during spermiogenesis. The mouse spermatid NL is composed of the ubiquitous lamin B1 and the spermatid-specific lamin B3, an N-terminally truncated isoform of lam...