Capturem Pepsin was developed for rapid and efficient digestion of antibodies—with a room-temperature protocol (Figure 1)—designed for downstream proteomics analysis. Pepsin is an acidic protease with utility in a wide variety of applications, most notably in antibody sample preparation for characterization by mass spectrometry (MS). The specificity (Keil, B. 1992) and activity of digestion are...

A second extracellular protease from myxobacter strain AL-1 has been purified to homogeneity and named protease II; the enzyme crystallizes as fine needles. The extracellular, cell wall lytic protease reported previously from the same organism is now designated protease I. Protease II exhibits a pH optimum of 8.5 to 9.0 and is stable from pH 3.0 to 9.0. The enzyme is heat stable at 50 C for 18 ...

The new model for the tertiary structure of ribosomal 5 S rRNA from plants recently proposed by some of us has been already supported by RNase H digestions in the presence of complementary oligodeoxynucleotides. These results are confirmed now by the new biochemical and NMR spectroscopy data. Diethylpyrocarbonate (DEP) and monoperphthalic acid (MPA) are the reagents with the high specificity to...

Bottom-up mass spectrometry-based protein analysis methods employing protease digestion are routinely used to identify and characterize proteins with high specificity sensitivity. Method performance is generally measured by sequence coverage capability the total number of characteristic peptides identified, when compared predicted databases. Limitations commonly solvent-based currently employed...

Article history: Received 5 October 2015 Received in revised form 17 October 2016 Accepted 21 October 2016 Available online 28 October 2016 Elevated CO2 and drought are key consequences of climate change and affect soil processes and plant growth. This study investigated the effects of elevated CO2 and drought on themicrobial biomass and enzymatic activities in the rhizospheres of Bothriochloa ...

Interstitial cells of Cajal (ICCs) are the pacemaker cells in the gastrointestinal tract, and histamine is known to regulate neuronal activity, control vascular tone, alter endothelial permeability, and modulate gastric acid secretion. However, the action mechanisms of histamine in mouse small intestinal ICCs have not been previously investigated, and thus, in the present study, we investigated...

The cytochemistry and morphology of interphase and con densed mitotic chromosomes are described in ultrathin-seetioned specimens prepared by freezing and drying, postfixation with protein-sensitive bifunctional reagents of low density, basophile staining with an electron-dense dye lake, and enzy matic digestions. In specimens not subjected to enzymes, interphase chromatinic threads were observe...

A gene encoding Trypanosoma brucei UDP-N-acetylglucosamine pyrophosphorylase was identified, and the recombinant protein was shown to have enzymatic activity. The parasite enzyme is unusual in having a strict substrate specificity for N-acetylglucosamine 1-phosphate and in being located inside a peroxisome-like microbody, the glycosome. A bloodstream form T. brucei conditional null mutant was c...