The proportions of glucose phosphate isomerase (GPI-1) allozymes produced by early (Gpi-1sa/Gpi-1sb female X Gpi-1sc/Gpi-1sc male)F1 mouse embryos were analysed by quantitative cellulose acetate electrophoresis. Technical controls showed that this system is extremely sensitive, quantitatively reproducible and quite accurate. Genetic controls established that the Gpi-1sa/Gpi-1sb mothers were hom...

Isozyme characters, exclusive of allozymes (alleles), are potentially useful in phylogenetic investigations. Such data have been derived from the multilocus L-lactate dehydrogenase (LDH) enzyme system. Among non-avian reptiles, the classes of potential characters include (1) and number of loci encoding LDH, (2) heterotramer patterns, (3) tissue-specific patterns of gene expression, (4) tissue-s...

Few genetic studies have been carried out in dolphinfish. Oxendorf & Hunte (1986a,b) compared samples obtained in the Caribbean Sea (Central-West Atlantic) using several physiological characters such as fecundity, growth or sexual maturity. The results obtained lead to the definition of two dolphinfish stocks in the Caribbean Sea. Later, the same samples were examined using allozymes. 16 out of...

Mitochondrial cytochrome b (cytb) is among the most extensively sequenced genes to date across the vertebrates. Here, we employ nearly 2,000 cytb gene sequences from GenBank to calculate and compare levels of genetic distance between sister species, congeneric species, and confamilial genera within and across the major vertebrate taxonomic classes. The results of these analyses parallel and rei...

Glucose phosphate isomerase (GPI) allozymes were compared in eggs and embryos of the mouse strains C57BL/6-JHan (GPI-1BB) and 129/Sv (GPI-1AA) under different experimental conditions. The quantitative differences in eggs of the two strains disappeared by the blastocyst stage at day 4 to 5, both in fertilized and diploid parthenogenetic embryos. The degree of degradation of oocyte-coded enzyme m...

Coltman and Slate (2003) recently performed a meta-analysis on studies that investigated the association between genetic variation at microsatellite loci and phenotypic trait variation. One factor not explicitly addressed in their meta-analysis is the actual estimation of genome-wide heterozygosity via molecular markers. Many authors still associate marker-estimated heterozygosity with genome-w...

Wild crop relatives are an important source of genetic variation for improving domesticated species. Given limited resources, methods for maximizing the genetic diversity of collections of wild relatives are needed to help spread protection over a larger number of populations and species. Simulations were conducted to investigate the optimal strategy of sampling materials from populations of wi...

Strictly defined, isozymes are similar forms of the same enzyme that share identical functions. There are different types of isozymes that are classified according to their origin isozymes may be encoded by several different loci (which determine subunit structure), or they may be determined by a single locus with several different alleles. If the latter, isozymes are referred to as "allozymes"...