background: green tea is one of the most popular beverages in the world. it is believed to have beneficial effects in the prevention and treatment of many diseases, one of which is nonalcoholic fatty liver disease (nafld). the present study investigated the effects of consumption of green tea in nafld patients. methods: this study was a double‑blind, placebo‑controlled, randomized clinical tria...

background: clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. however, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. this study was performed to investigate whether...

background: hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albendazole (abz) and mebendazole (mbz). the purpose of this study was comparing the effects of abz and mbz on glutathione-s-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst. methods: the culture supernatants containing the parasite excretory...

the role of parathyroid hormone on stature in major beta-thalassemic patients   gr. moshtaghi kashanian phd 1 , a. ahmadi md2, mm. hayat bakhsh md3   1- assistant professor, dept. of biochemistry, faculty of medicine, university of medical sciences, kerman, iran   2- professor of pediaterics, university of medical sciences, kerman, iran   3- assistant professor of internal medicine, faculty of ...

background : colorectal cancer, a major health concern worldwide, is the third most common form of cancer and second leading cause of cancer-related deaths. the flavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods and important components of the human diet. luteolin, a bioflavonoid, possesses many beneficial effects including antioxidant, anti-inflammatory, anti-aller...

The effect of Tris, Glycine and Tricine on alkaline phosphatase activity was tested at a pH of 8.6, 8.85 and 9.1, using pNPP as substrate. Alkaline phosphatase activity was measured by monitoring the increase in NP absorbance at 420nm using a spectrophotometer. Alkaline phosphatase in Tris buffer at pH 9.1 was found to have the highest activity. MichaelisMenten enzyme kinetic analysis revealed ...

OBJECTIVE To determine whether serum vitamin D levels are correlated with serum levels of alkaline phosphatase or not. STUDY DESIGN Cross-sectional, observational study. PLACE AND DURATION OF STUDY Multi-centre study, conducted at Liaquat National Hospital and Medical College, National Medical Centre and Medicare Hospital, Karachi, from January to October 2009. METHODOLOGY Patients attend...

uridine (lUdR) there was an increase in alkaline phosphatase activity after about 24 hr, reaching a maximum usually at about 72 hi. At this concentration, during this time, there was a relatively small inhibition of cell replication. The increment in alkaline phosphatase activity was in large measure prevented by concomitant addition of 10 /¿Mthymidine. Further, lUdR did not increase alkaline ...

Ewen, Lilian M.: Separation of alkaline phosphatase isoenzymes and evaluation of the clinical usefulness of this determination. Am. J. Clin. Pathol. 61: 142-154, 1974. A simple technic for separation of alkaline phosphatase isoenzymes on agarose gel, using thymolphthalein monophosphate as the isoenzyme indicator substrate, is described. Results demonstrate that separation of isoenzymes of alkal...

Alkaline phosphatase serves both as a model enzyme for studies on the mechanism and kinetics of phosphomonoesterases and as a reporter in enzyme-linked immunosorbent assays (ELISAs) and other biochemical methods. The tight binding of the enzyme to its inorganic phosphate product leads to strong inhibition of catalysis and confounds measurements of alkaline phosphatase activity. We have develope...