We studied the effects of extracellular pH (pHo) on gamma-aminobutyric acid (GABA)-mediated Cl- current in rat hypothalamic neurons and recombinant type-A GABA (GABA(A)) receptors stably expressed in human embryonic kidney cells (HEK 293), using whole cell and outside-out patch-clamp recordings. In alpha3beta2gamma2s receptors, acidic pH decreased, whereas alkaline pH increased the response to ...

Using the distribution of weak acids to measure the pH gradient (delta pH; interior alkaline) and the distribution of the lipophilic cation [3H]tetraphenylphosphonium+ to monitor the membrane potential (delta psi; interior negative), we studied the electrochemical gradient or protons (delta mu- H+) across the membrane of Micrococcus lysodeikticus cells and plasma membrane vesicles. With reduced...

Alkaliphilic bacteria typically grow well at pH 9, with the most extremophilic strains growing up to pH values as high as pH 12-13. Interest in extreme alkaliphiles arises because they are sources of useful, stable enzymes, and the cells themselves can be used for biotechnological and other applications at high pH. In addition, alkaline hydrothermal vents represent an early evolutionary niche f...

IT is widely accepted that the physiological activity of alkaloidal eye preparations results from the penetration through the cornea of the free base portion rather than of the ionic form of the alkaloid (Cogan and Kinsey, 1942; Cogan and Hirsch, 1944; Swan and White, 1942). Therefore, the smaller the pKb value of the alkaloid, the fewer free base particles will be available for drug action at ...

Airways secrete considerable amounts of acid. In this study, we investigated the identity and the pH-dependent function of the apical H(+) channel in the airway epithelium. In pH stat recordings of confluent JME airway epithelia in Ussing chambers, Zn-sensitive acid secretion was activated at a mucosal threshold pH of approximately 7, above which it increased pH-dependently at a rate of 339 +/-...

To fully understand the glycolytic behavior of cancer cells, it is important to recognize how it is linked to pH dynamics. Here, we evaluated the acute effects of mild acidification and alkalization on cancer cell glucose uptake and glycolytic flux and investigated the role of hexokinase (HK). Cancer cells exposed to buffers with graded pH were measured for 18F-fluorodeoxyglucose (FDG) uptake, ...

The degree of inhibition of rat intestinal alkaline phosphatase by L-phenylalanine was highly pa-dependent and varied from 0 to 66% within a pH range of ‘7.8 to 10.4, exhibiting a peak at pH 9.2 and 8.7 for phenylphosphate and /3glycerophosphate, respectively. Vm,, was also a function of pH with and without the inhibitor. Rat intestinal alkaline phosphatase exhibited maximum enzyme activity at ...

The degree of inhibition of rat intestinal alkaline phosphatase by L-phenylalanine was highly pa-dependent and varied from 0 to 66% within a pH range of ‘7.8 to 10.4, exhibiting a peak at pH 9.2 and 8.7 for phenylphosphate and /3glycerophosphate, respectively. Vm,, was also a function of pH with and without the inhibitor. Rat intestinal alkaline phosphatase exhibited maximum enzyme activity at ...

The aim of this study was to evaluate the effect of alkaline and NaCl on functional properties of protein isolated from Lanternfish (Benthosema pterotum). In this study, extraction of fish protein was performed at different concentrations of NaCl (0.5%, 1.5%, 3% and 4.5%) at pHs 10 and 12. The results showed alkaline treatment improved the functional properties and the recovery yield of protein...