Bacteriological laboratories engaged in diagnostic or research problems concerning pathogenic enteric bacilli, namely, the Salmonella and Shigella groups, invariably find that members of the genus Proteus are by far the most misleading. A dependable, easily interpreted, routine differential test medium for screening the latter group of bacilli becomes desirable. Urease production is a character...

Periodontium is a complex organ composed of mineralized epithelial and connective tissue. Dexamethasone could stimulate proliferation of osteoblast and fibroblasts. This study aimed to assess the osteogenic effect of dexamethasone on periodental ligament (PDL) stem cells. PDL stem cells were collected from periodontal ligament tissue of root of extracted premolar of young and healthy people. Th...

Abstract Synthesis of water‐soluble‐substituted pyridinium cation which is attached with base sensitive fluorophore peripheral units different inorganic counter anions under conventional/silica‐supported Muffle furnace methods. Solvent‐free synthetic approach has more merits than the conventional method, such as non‐toxic, shorter reaction time, easy workup procedure without purification proces...

Alkaline proteases producing halophilic bacteria isolated from Sambar Lake, Rajasthan were investigated for keratinolytic activity. The aim of this study was to optimize culture conditions for maximum enzyme production as well as characterization of proteases with keratinolytic action. Casein was used as sole carbon and nitrogen source for alkaline protease production at 37°C and 150 rpm. Highe...

Proteolytic bacteria are most important for industries such as food and fermentation. Thus isolating and manipulating pure culture from various sources has importance for various biotechnology industries. In the present investigation a total of 26 bacteria were isolated from the dairy plant effluent. All isolates were screened for proteolytic activity using alkaline skim milk agar plate, among ...

Lactate dehydrogenase and alkaline phosphatase activities in the same medium can be determined simulataneously, at 350 and 550 nm, with a vidicon spectrometer. Substrate concentrations and ph have been made optimum for the combined analysis. These conditions result in activities for lactate dehydrogenase that are equivalent to those found by methods in common use, and activites for alkaline pho...

Numerous alkalotolerant and alkalophilic bacteria, with concentrations ranging from 10 to 103 cells 100 ml-' water sample, were isolated from seawater in the Pacific and Indian Oceans using a buffered medium of pH 10.6. The pH effects on growth rates were used to define the occurrence of three types of alkalotolerant and alkalophilic bacterial groups: (1) bacteria exhibiting high growth rates t...

Optimization of the fermentation medium for maximum alkaline protease production was carried out. Fifteen positive isolates were examined for their extent of alkaline protease production. The most potent producer was identified as Bacillus sp. The solid substrate screening showed that the combination of wheat straw and bean husk was the best one. The initial screening by using Plackett–Burman’s...

The hemolytic activity of Serratia marcescens was examined as a function of iron availability. Restriction of iron by the nonmetabolizable chelator 2,2'-dipyridyl or the iron-binding protein transferrin produced a marked increase in hemolytic activity. The hemolytic activity of S. marcescens is determined by two adjacent genes, 5'-shlB-shlA-3', where shlA encodes the hemolysin which requires th...