PURPOSE To investigate the use of atomic force microscopy (AFM) to image the three groups of corneal epithelial cells fractionated by a novel rapid centrifugation isolation technique. METHODS Epithelial cells harvested from primary cultures of rabbit limbal rings were centrifuged onto uncoated dishes, first at 1400 rpm and then at 1800 rpm. The adherent cells after centrifugation at 1400 rpm ...

The proposed work presents the results for contact angle and atomic force microscopy (AFM), which are applied to characterize surface of two types samples, “reference” sample “space” sample, both made same material – graphite covered with glassy carbon coating, but stored 28 months in different environments (conditions). One was mounted outside International Space Station, other on Earth.
...

Spray pyrolysis technique (SPT) and radio-frequency magnetron sputtering (RF-MS) were used to obtain vanadium oxide (VxOy) layers. The surface morphology was visualized using scanning electron microscopy (SEM) atomic force (AFM). Further, t

nanostructured alumina thin films were coated on stainless steel by sol-gel dip coating method. in order to prevent crack formation, al2o3 films were kept in a solvent bath immediately after coating to reduce the rate of drying. effects of calcination temperature and withdrawal speed on structural properties were analyzed using xrd and sem. topography and thickness of coatings were analyzed by ...

We present a detailed study of the interface morphology of an electro-deposited (ED) Ni/Cu bilayer film by using off-specular (diffuse) neutron reflectivity technique and Atomic Force Microscopy (AFM). The Ni/Cu bilayer has been electro-deposited on seed layers of Ti/Cu. These two seed layers were deposited by magnetron sputtering. The depth profile of density in the sample has been obtained fr...

Riboflavin receptors are overexpressed in malignant cells from certain human breast and prostate cancers, and they constitute a group of potential surface markers important for cancer targeted delivery of therapeutic agents and imaging molecules. Here we report on the fabrication and atomic force microscopy (AFM) characterization of a core-shell nanocomposite consisting of a gold nanoparticle (...

Mixed Langmuir and Langmuir-Blodgett films of dissimilar components, arachidic acid and a phthalocyanine of zinc, have been investigated. These compounds form mixed films with phase separation, with domains at the sub-micro scale which are detected by AFM in LB films but not observed by BAM. These domains present different contrast in the surface potential images obtained with SP-AFM and in the...

Atomic force microscopy (AFM) has become an important tool for quantifying mechanical properties of biological materials ranging from single molecules to cells and tissues. Current AFM techniques for measuring elastic and viscoelastic properties of whole cells are based on indentation of cells firmly adhered to a substrate, but these techniques are not appropriate for probing nonadherent cells,...

background: aflatoxin m 1 (afm 1 ) is the metabolite of aflatoxin b1 (afb 1 ) and is found in milk when lactating animals are fed with contaminated feedstuff.  the presence of afm 1 in milk, pose a major risk for humans especially kids as it can have immunosuppressive, mutagenic, teratogenic and carcinogenic effects. the present study is aimed to investigate the oc­currence of afm 1 in subsidiz...

It has been suggested that Alba, a well-established chromatin protein in Archaea, is also a subunit of the archaeal RNase P holoenzyme, based on the observation that the homolog of this protein in humans has been shown to be associated with RNase P activity. Using the same biochemical methods we used previously to show that four other proteins homologous to eukaryotic RNase P proteins are bona ...