The periapical microbiota of 36 teeth with refractory apical periodontitis was investigated. None of the teeth had responded to conventional endodontic or long-term (> 6 months), calcium-hydroxide treatment. Eight patients had received antibiotics systemically. After anaerobic culture, a total of 148 microbial strains were detected among 67 microbial species. One of the 36 lesions was culture-n...

Tyrosylprotein sulfotransferase (TPST), responsible for the sulfation of a variety of secretory and membrane proteins, has been identified and characterized in submandibular salivary glands (William et al. Arch Biochem Biophys 1997; 338: 90-96). In the present study we demonstrate the sulfation of a salivary secretory protein, statherin, by the tyrosylprotein sulfotransferase present in human s...

This in vitro study evaluated the seal created by varying depths of mineral trioxide aggregate (MTA) plugs placed in an orthograde fashion in five groups of 10 teeth. One group received a 2 mm thick orthograde apical plug of MTA, the second group a 5 mm apical MTA plug, and the third group a 2 mm apical MTA plug with a second 2 mm increment, 24 h later. The remaining portion of the canal in the...

A soluble wall-associated common protein (WAP) has been isolated from cell wall-extracted antigens of exponentially growing cells of Streptococcus sanguis OMZ9, two serotypes (a and e), of Streptococcus mutans and Actinomyces viscosus OMZ104. The WAP antigens from the different strains were obtained by chromatography on an anti-rabbit immunoglobulin column of sodium dodecyl sulfate-dissociated ...

actinomycosis is an indolent, slowly progressive infection caused by anaerobic or microaerophilic bacteria, primarily of genus actinomyces, which colonize the mouth, colon and vagina. mucosal disruption may lead to infection virtually at any sites in the body. the aim of this study was to underline different features of actinomycosis and to represent total data about etiologic agents, clinical,...