نتایج جستجو برای: 80 rapd primers were employed for pcr reactions
تعداد نتایج: 11188208 فیلتر نتایج به سال:
This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not ...
Aeromonas hydrophila isolated from fish (Labeo rohita), pond water, river water, raw meat of chicken and mutton and raw cow milk were characterized through Random Amplified Polymorphic DNA (RAPD) analysis and Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE) of cellular proteins. RAPD-PCR amplification of bacterial DNA was done by using ten random primers (OPA-01 to 10) and ...
The trematode, Stellantchasmus falcatus, is one of the members in the Family Heterophyidae, which is reported to be an endemic fluke in northern region of Thailand. A sensitive and specific detection of this parasite is required to determine the epidemiology at larval stages. Specific primers to determine the presence of S. falcatus were investigated using high annealing temperature random ampl...
the purpose of this study was to examine the english language needs of medical students at tehran university of medical sciences . analysis of the needs took place for three groups: 320 undergraduate students, 30 postgraduate students and 20 university instructors. a triangulation approach to collect data was used in which a combination of the quantitative (using the questionnaires) and qualita...
The common fig (Ficus carica L.), one of the most important fruit species, belongs to Moraceae family and is widely distributed in Iran. In this study, genetic variations among some genotypes of common fig collected from six different regions of Ilam province (Iran) were evaluated based on RAPD and ISSR markers. A total of 73 and 29 alleles were produced by 14 RAPD (with their sizes ranging fro...
the following null hypothesis was proposed: h : there is no significant difference between the use of semantically or communicatively translates scientific texts. to test the null hypothesis, a number of procedures were taken first, two passages were selected form soyrcebooks of food and nutrition industry and gardening deciplines. each, in turn, was following by a number of comprehension quest...
In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...
The purpose of this study was to type the Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates recovered from cystic fibrosis (CF) patients by random amplified polymorphic DNA (RAPD)-PCR and to determine the antibiotic susceptibility of these strains. P. aeruginosa (n=49), and S. maltophilia (n=11) isolates which had been recovered from 16 and 8 patients, respectively, during a 1-ye...
Genetic variations of 15 Brahmi (Bacopa monnieri L.) accessions were evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. During RAPD analysis, amplification of genomic DNA of the 15 accessions by 22 primers generated 197 fragments, of which 187 were polymorphic with an average of 8.95 bands per primer. The amplified products varied in size f...
The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), consistently appearing when genomic DNA was amplified with an aspecific primer (OP...
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