Interferon gamma (IFN-γ) is one of the key cytokines in defining T helper 1 lymphocyte immuneresponses. In this study, the bovine IFN-γ gene was cloned from spleen tissue RNA using the reversetranscription-polymerase chain reaction (RT-PCR). IFN-γ cDNA was sub-cloned and expressed inmammalian expression plasmid (pcDNA3.1(+)) under the control of the human cytomegalovirus (CMV)promoter. The pred...

conclusions large amounts of preclinical studies have been made of stem cells and there is an increasing interest to perform these studies within the human population but preclinical studies are insufficient; therefore, much more and efficient studies should be conducted to evaluate the efficacy and safety of stem cells. context musculoskeletal injuries may be painful, troublesome, life limitin...

Introduction: A main choice for cancer treatment is radiotherapy. But, the radiotherapy disadvantage is damages caused by radiation given to normal tissues/organs surrounding cancer. One way to avoid this is via increasing radiosensitization of cancer cells. Gold nanoparticles (GNPs) have shown sensitizing effect on cancer cells by enhancing their absorbed dose. Unlike earlier ...

background: several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (mscs) in allogeneic or mitogenic interactions. cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. objective: to investigate if adipose derived mscs could inhibit jurkat lym-phoblastic leukemia t cell proliferation during coculture. me...

age related cytochemical changes of thymic endocrine cells were studied in 78 day old chicks at five day interval to age of day 60 employing a panel of cytochemical stains. methenamine silver revealed cell morphology including cell processes distinctly while diamine silver revealed a stronger argentaffinity in these cells. the cells had greater affinity for diamine silver compared to methenamin...

Introduction: The G-protein coupled receptor 30 (GPR30) gene is a member of the G-protein coupled receptor (GPCR) family; involved in breast, endometrial, and ovarian cancers. Many GPCR receptors that are implicated in several types of human cancers are correlated with increased cell proliferation and tumor progression; especially GPR30 gene. Methods: The breast cancer MCF-7 and MDA-MB-231 cel...

Abstract: Coxsackievirus B3 (CVB3) is the most common agent known to cause viral myocarditis. The viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. Most of this proteolytic processing is catalyzed by a cysteine protease called 3C. The 3C protease plays major role in viral replication and cellular damage. To understand the mecha...

chamaemelum nobile (asteraceae) commonly known as 'roman chamomile' is a medicinal plant used for numerous diseases in traditional medicine, although its anticancer activity is unknown. the present study was carried out to investigate the anticancer as well as apoptotic activity of ethyl acetate fraction of c. nobile on different cancerous cell lines. the cells were treated with varyi...

Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lin...

sirtuin1 (sirt1) is an enzyme that deacetylates histones and several nonhistone proteins including p53 during stress and plays an important role in the survival of tumor cells. hereby, this study describes the potency of salermide as a sirt1 inhibitor to induce apoptosis in the mcf-7 and mrc-5 cell lines. mcf7 and mrc-5 cell lines were cultured in rpmi-1640 and treated with or without salermide...