Resistance to extended-spectrum β-lactam antibiotics has led to a greater reliance upon carbapenems, but the expression of carbapenemases threatens to limit the utility of these drugs. Current methods to detect carbapenemase activity are suboptimal, requiring prolonged incubations during which ineffective therapy may be prescribed. We previously described a sensitive and specific assay for the ...

The objective of our study was to describe the molecular support of carbapenem resistance from randomly selected clinical isolates of multidrug-resistant (MDR) Acinetobacter baumannii as a pilot study from the Hamad Medical Corporation (HMC), Qatar. Results of our report will be used to study carbapenemases using molecular techniques in all isolated MDR A. baumannii. Forty-eight MDR A. baumanni...

BACKGROUND Multidrug-resistant organisms (MDRO) are a worldwide problem. International migration and travel facilitate the spread of MDRO. Therefore the goal of our study was to assess the risk of influx of MDRO from patients transferred to one of Central Europe's largest hospitals from abroad. METHODS A mono-centre study was conducted. All patients transferred from other countries were scree...

tions. To allow the database to reflect the content of the live repositories to within a useful time frame, any regular automated updates of the reference database from live repositories could label new alleles with ‘provisional’ designations prior to committee approval. As a method for identifying resistance genes from WGS data the use of a reference pseudomolecule/database may yet be supersed...

Background Reports of nosocomial infection due to carbapenem resistant Serratia spp. have become significantly more common. This resistance may be due to production of distinct carbapenemases, such as KPC [1,2]. This enzyme, initially described in Klebsiella pneumoniae isolates, has also been detected among other organisms, such as Serratia marcescens, emphasizing the global risk of interspecie...

The first case of carbapenemase-producing Enterobacteriaceae in Italy was reported in 2009. We performed a study over a period of seven months in 2010 to survey the circulation of Klebsiella pneumoniae carbapenemases (KPC) ina 1,500-bed university hospital in northern Italy and report the presence and rapid increase of these multidrug-resistant bacteria. The results raise a major concern about ...

A collection of 15 carbapenem-resistance Acinetobacter baumannii clinical isolates was analysed on two tertiary hospitals in Mexico. The OXA-51 was identified in all isolates, followed by OXA-239 and OXA-58; OXA-239 is described as a new OXA-23-like allele. These carbapenemases were identified on four clonal groups, distributed between two neighbouring hospitals. Acinetobacter baumannii is poor...