Fusarium verticillioides (teleomorph Gibberella moniliformis) is a pathogen of maize worldwide and produces fumonisins, a family of mycotoxins that have been associated with several animal diseases as well as cancer in humans. In this study, we sought to identify fungal genes that aVect fumonisin production and/or the plant–fungal interaction. We generated over 87,000 expressed sequence tags fr...

OBJECTIVES The purpose of this study was to evaluate the effect of Cuminum cyminum (C. cyminum) essential oil on the growth and FUM1 gene expression of fumonisin-producing Fusarium verticillioides (F. verticillioides) strains isolated from maize. MATERIALS AND METHODS All fungal strains were cultured on potato dextrose agar (PDA) slopes at 30°C for 7 days. The antifungal activity was evaluate...

The fumonisins, a family of food-borne carcinogenic mycotoxins, were first isolated in 1988 (21) from cultures of Fusarium verticillioides (Sacc.) Nirenberg (previously known as Fusarium moniliforme Sheldon). During the same year, the structures of the fumonisins were elucidated (6) and fumonisin B1 was shown to cause equine leukoencephalomalacia (34). There have been numerous publications deal...

A simple method for analysis of fumonisin B1 (FB1) and fumonisin B2 (FB2) in beer and raw materials of beer using liquid chromatography-electrospray ionization tandem mass spectrometry (LC/MS/MS) was developed. Samples were prepared and purified by using solid-phase extraction columns (SAX). The LC separation was performed with an octadecylated silica column at a flow-rate of 0.2 mL/min, using ...

Fumonisins are mycotoxins found in maize. In developing countries, the resources required for analysis are often lacking, and the shipping of maize between countries can be difficult since the importation of plant materials requires permits/inspection to prevent the entry of pests that frequently infest maize. A simple, safe and legal method for shipping maize extracts to the USA was needed to ...

Oxidative stress, which is characterized by disruption of the oxidant/antioxidant balance, causes pathological processes, including toxicities induced certain mycotoxins. The present study was designed to investigate effects resveratrol on sirtuin deacetylases (SIRT2 and SIRT3), nitric oxide (NO), reduced glutathione (GSH) malondialdehyde (MDA) in fumonisin B1-induced hepatotoxicity. Regarding ...

The investigation was aimed to evaluate the antifungal and antifumonisin activities of budmunchiamine A and pithecolobine against Fusarium verticillioides. The budmunchiamine A was isolated from Albizia amara and pithecolobine from Albizia saman. The results demonstrated that both budmunchiamine A and pithecolobine significantly inhibited the growth and fumonisin B1 production by F. verticillio...

Fumonisins are mycotoxins found in maize. In developing countries, the resources required for analysis are often lacking, and the shipping of maize between countries can be difficult since the importation of plant materials requires permits/ inspection to prevent the entry of pests that frequently infest maize. A simple, safe and legal method for shipping maize extracts to the USA was needed to...

Mycotoxin contamination levels in maize kernels are controlled by a complex set of factors including insect pressure, fungal inoculum potential, and environmental conditions that are difficult to predict. Methods are becoming available to control mycotoxin-producing fungi in preharvest crops, including Bt expression, biocontrol, and host plant resistance. Initial reports in the United States an...

The effects of feeding Fusarium moniliforme culture material, containing known concentrations of fumonisin Bj (FBi), were studied in turkey poults. Day-old poults were allotted randomly to dietary treatments containing 0, 0.41, 0.82, 1.23, 1.64, 2.87, 4.10, 5.33, 6.56, and 7.79% fumonisin culture material (FCM). These levels of FCM supplied 0, 25, 50, 75, 100, 175, 250, 325, 400, and 475 mg FBi...