S.P.L. Sgrensen not only introduced the p H concept, but in his classical paper from 1909 “Enzymstudien 11” he also described in great detail the colorimetric as well as the potentiometric methods for p H measurement, and he pointed out that two kinds of acidity must be distinguished: Actual acidity and titratable acidity. Previous to Sgrensen the acidity of a solution was usually determined by...

BACKGROUND Septic shock is a common and often devastating syndrome marked by severe cardiovascular dysfunction commonly managed with vasopressors. Whether markers of heart rate complexity before vasopressor up-titration could be used to predict success of the up-titration is not known. METHODS We studied patients with septic shock requiring vasopressor, newly admitted to the intensive care un...

Objective: Garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. Infectious bronchitis virus (IBV) is a coronavirus. The available vaccines against IBV cannot cover new variants. This study evaluated the inhibitory effects of garlic extract on IBV.  Materials and Methods: The constituents of garlic extract were detected by gas chromatography. This study was d...

The most commonly used technique for water content determination is Karl-Fischer titration with electrometric detection, requiring specialized equipment. When appropriate equipment is not available, the method can be performed through visual detection of a titration endpoint, which does not enable an analysis of colored samples. Here, we developed a method with spectrophotometric detection of a...

NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...

An enzyme-linked immunosorbent assay (ELISA) was developed on the basis of testing a single dilution of serum to quantify the level of antibody to the p57 protein of Renibacterium salmoninarum in sockeye salmon (Oncorhynchus nerka). The levels of antibody were interpolated from a standard curve constructed by relating the optical densities (OD) produced by several dilutions of a high-titer rain...

Calorimetric titrations of glycogen phosphorylase b (EC 2.4.1.1) from rabbit muscle with its allosteric activator, AMP, have been carried out at 25 “C and pH 6.9 in three different buffer systems (glycylglycine, glycerophosphate, and tris(hydroxymethy1)aminomethane). Calorimetric curves of biphasic nature were originally obtained. This biphasic behavior was always concomitant with: (a) a depen...