Background Currently, stirred tank reactors (STR) represent the gold standard for the large scale growth of suspension cell lines. Culture performance is strongly influenced by the efficiency of mixing, as measured by the mass transfer coefficient and mixing time. The success of traditional stainless steel or glass STR systems lies in their impeller driven agitation that efficiently mixes large...

Embryonic stem (ES) cells can differentiate into functional hepatic lineage cells, which can potentially be used in biomedicine. To obtain hepatic lineage cells from ES cells, embryoid bodies (EBs) must be formed. In this study, we developed an EB formation system using a spinner flask for mass production of EBs. ES cells were inoculated into the spinner flask, where they formed EBs within 4 d....

Problem Statement: Mixing filamentous fungi entails delicate balance between promoting high volumetric oxygen transfer coefficient (kLa) while keeping low hydrodynamic stresses in the microenvironment of cultures. This study examined the oxygen transfer capability of a prototype low shear helical impeller as a potential replacement for the standard Rushton turbine. Approach: The dependence of k...

Stirred-suspension bioreactors are a promising modality for large-scale culture of 3D aggregates of pluripotent stem cells and their progeny. Yet, cells within these clusters experience limitations in the transfer of factors and particularly O2 which is characterized by low solubility in aqueous media. Cultured stem cells under different O2 levels may exhibit significantly different proliferati...

SARS-CoV-2 was identified as the pathogenic agent causing COVID-19 pandemic. Among proteins codified by this virus, Spike protein is one of most-external and -exposed. A fragment protein, named receptor binding domain (RBD), interacts with ACE2 receptors human cells, allowing entrance viruses. RBD has been proposed an interesting for development diagnosis tools, treatment, prevention disease. I...

Introduction Disposable bioreactors are increasingly gaining acceptance for cell culture applications due to a number of advantages including ease of use and reduced labour costs, less requirements for utilities such as steam and purified water. In addition, the system requires no cleaning or cleaning validation and only a reduced clean room footprint. Integrated ready to use pre-sterilised dis...