BACKGROUND Cell mediated immunity is suppressed following traumatic injury. The Objective is to determine whether there is a shift from T helper type 1 (TH1) to TH2 cell cytokine production following mechanical trauma in a rat model. METHODS Male Sprague-Dawley rats were anesthetized and subjected to bilateral femur fractures or sham injury. Spleens were removed 3 days later. T cell prolifera...

Adult Mice (BWt. 30 gms and 40gms) were treated at a 5mg\kgBWt. and 10 mg/kg BWt. of Ethidium Bromide for 10 days in drinking water. Control animals were given equal dose of deionised water. Quantitative and qualitative changes were studied in Spleen. The following features were evaluated: the overall cellularity of the spleen was noticed. The changes were characterised by, injury to spleen tis...

A monoclonal antibody against T200 glycoprotein is selectively cytotoxic for thymocytes and mature thymus-dependent (T) lymphocytes. All T-cell functions assayed, cell-mediated cytotoxicity, helper cell activity, and proliferation in response to T-cell mitogens orallogeneic cells were abolished by prior treatment of spleen cells with anti-T200 antibodies and complement. In contrast, thymus-inde...

Mitochondrial uncoupling protein 3 (UCP3) is constitutively expressed in mitochondria from thymus and spleen of mice, and confocal microscopy has been used to visualize UCP3 in situ in mouse thymocytes. UCP3 is present in mitochondria of thymus and spleen up to at least 16 weeks after birth, but levels decrease by a half in thymus and a fifth in spleen after three weeks, probably reflecting the...

Background: Lymphoma arises in lymph nodes. Infiltration of malignant lymphomatous cells the organs other than node is termed as extranodal lymphoma (ENL). Almost any organ body can get affected. The most frequently involved system gastrointestinal tract, followed by lung, liver, spleen, bone, and skin. Primary central nervous (CNS) also well documented. Origin tumor from non-lymph-nodal tissue...

Propoxure (PPX) is a well-known carbamate insecticide, which has been used for several decades in the world and Iran in agriculture and public health programs. However, there is no clear investigation toward its immunotoxicity as yet. In this study, we examined the effects of subchronic i.p. exposure of PPX on humoral (PFC & HA) and cellular (DTH) responses, and also monitored T-Cell subtypes u...

propoxure (ppx) is a well-known carbamate insecticide, which has been used for several decades in the world and iran in agriculture and public health programs. however, there is no clear investigation toward its immunotoxicity as yet. in this study, we examined the effects of subchronic i.p. exposure of ppx on humoral (pfc & ha) and cellular (dth) responses, and also monitored t-cell subtypes u...

Autoimmunity and inflammation are controlled in part by regulatory B cells, including a recently identified IL-10-competent CD1d(high)CD5(+) B cell subset termed B10 cells that represents 1-3% of adult mouse spleen B cells. In this study, pathways that influence B10 cell generation and IL-10 production were identified and compared with previously described regulatory B cells. IL-10-competent B ...

Considering the emerging importance of phenotypic markers as indicators of cell function and differentiation, we studied patterns ofCD44 and CD45RB expression in CD8+ murine T cells with prior exposure to antigen or staphylococcal enterotoxin B ( SEB ). Following in vivo priming with two purified protein derivatives (one from a virulent WHO strain and the other from an avirulent strain), T ...

Hematopoietic cell lineages are best described in terms of distinct progenitors with limited differentiative capacity. To distinguish cell lineages, it is necessary to define progenitors and induce their differentiation in vitro. We previously reported in vitro development of immature dendritic-like cells (DCs) in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or bone m...