Currently, the assessment of sperm function in a raw or processed semen sample is not able to reliably predict sperm ability to withstand freezing and thawing procedures and in vivo fertility and/or assisted reproductive biotechnologies (ART) outcome. The aim of the present study was to investigate which parameters among a battery of analyses could predict subsequent spermatozoa in vitro fertil...

We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejac...

BACKGROUND Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the seme...

This study examines the effect of varying several factors, both extrinsic and technology-dependent, on the reconstruction of human sperm trajectories and the derived kinematic measurements using videotapes and the Motion Analysis Celltrak/S instrument. In semen samples from normal healthy men, curvilinear (VCL) and straight line velocities (VSL) were found to increase 1.5-fold, and linearity (L...

Juwet fruit is high antioxidants that can increase reproductive ability. This study aims to see the effect of juwet extract on morphology and ratio X Y spermatozoa after exposure cigarette smoke. used a completely randomized design (CRD) with 5 treatments namely K-, K+ (given 1 commercial cigarette/day), P1 1.8 mg/g BW one P2 ( given BW) P3 filter/day) each treatment had 4 replications. The par...

Biomarker-based sperm analysis elevates the treatment of human infertility and ameliorates reproductive performance in livestock. The negative biomarker-based approach focuses on proteins and ligands unique to defective spermatozoa, regardless of their morphological phenotype, lending itself to analysis by flow cytometry (FC). A prime example is the spermatid specific thioredoxin SPTRX3/TXNDC8,...

Human sperm samples were prepared on a 30% Percoll gradient and reactive oxygen species (ROS) production was measured. In samples that generated ROS incubation under 95%O2:5%CO2 for 30 min decreased the proportion of spermatozoa capable of the stimulated acrosome reaction by 40% in comparison to samples incubated under 95%N2:5%CO2 (P< 0.001, repeated measures analysis of variance), but the degr...

The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, in vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30 degrees C or in liquid nitrogen vapour (LNV)]. For the in vivo trial, females were inseminated wit...

high ambient temperature is the major constraint on buffalo productivity. the aim of this study was to evaluate the reproductive performance and thyroid gland function in winter and summer seasons in khuzestan buffalo bulls. six male indigenous buffaloes of khuzestan with nearly the same age (2-3 years old) and weight were used. semen and blood samples through jugular vein were collected, every...

Human spermatozoa are capable of a carrier-mediated gamma-aminobutyric acid (GABA) uptake. The uptake is dependent on the concentration of Cl- and Na+ in the external medium, and the kinetics of the carrier resembles high-affinity GABA transport proteins. The time-dependent uptake of GABA displays large interindividual differences and is not correlated to motility parameters or morphology in th...