Fluorescent protein-based pH sensors are useful tools for measuring protein trafficking through pH changes associated with endo- and exocytosis. However, commonly used pH-sensing probes are ubiquitously expressed with their protein of interest throughout the cell, hindering our ability to focus on specific trafficking pools of proteins. We developed a family of excitation ratiometric, activatab...

Sparingly soluble Ion—association compounds Thrmed between multicharged anionic complexes ( formed from trace amounts of an element to be preconoentrated and determined) and hydrophobic basic dyes, accumulate at the phase boundary after shaking with a solvent of low polarity, This type of flotation (without surfactant and gas bubbling) depends on the acidity, the dyestuff and the flotation agen...

with the development of beverage industry and expansion of the markets , packaging of these products has also come out of its traditional form and intelligent packaging has been applied gradually. different types of smart packaging are used in the beverage industry such as gas release packaging which by gas production causes a good texture and is used in order to create a creamy milk coffee. in...

To overcome the limited brightness of existing fluorogenic molecular probes for biomolecular targets, we introduce a concept dendrimer probe, which undergoes polarity-dependent switching due to intramolecular aggregation-caused quenching its fluorophores. Based on rational design dendrimers with four and eight squaraine dyes, found that octamer bearing dyes through sufficiently long PEG(8) link...

Levels of intracellular Ca2+ were monitored using fluorescence from Ca2+-sensitive dyes in chick embryonic heart cells cultured in an annular geometry. There was spontaneous starting and stopping of reentrant waves of activity. The results are modeled using modified FitzHugh-Nagumo equations representing pacemakers embedded in a conducting medium. These results provide a potential mechanism for...

A new method to label and detect the long ssDNA products from rolling circle (RC) amplification was reported. ZnS nanocrystal clusters (NCCs) were used to tag the RC products. The NCCs release millions of cations to trigger millions of metal-responsive dyes for cascade signal amplification. Selective and sensitive quantification of miRNA was demonstrated; and the NCCs delivered much lower detec...

In this paper we present an optogenetic neural recording system and its application to the study of the mouse visual cortex. We used a 32 x 32 fast and custom-designed imaging chip and system specifically designed to operate with voltage sensitive dyes. The system can operate up to 1000 frames per second, delivering a peak SNR performance of 63 dB, weighs less than 10 grams and consumes 12 mW o...

Azo dyes are widely spread in our day life, being heavily used cosmetics, healthcare products, textile industries, and as artificial food colorants. This intense industrial activity, which inherently includes their own production, inexorably leads to uncontrolled release of into the environment. As emerging pollutants, detection, particularly water systems, is a priority. Herein, fluorescence-b...

An efficient, sensitive and fast method was developed based on an ultrasound-assisted extraction followed by an ion-pair dispersive liquid-liquid microextraction (USAE-IP-DLLME) for the simultaneous determination of five commonly used synthetic sulfonate dyes (tartarazine, quinoline yellow, sunset yellow, azorubine, and brilliant blue) in ice cream samples using high performance liquid chromato...

Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with affinities in the ra...