The expression “metabolic reprogramming” has been encountered more and in the literature since mid-1990s. It seems to encompass several notions depending on author, but lack of a clear definition allows it be used as “catch-all” expression. Our first intention is point out inconsistencies use reprogramming terminology for cancer metabolism. second address over-focus role mutations metabolic ada...

Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human from dermal fibroblasts healthy females using Sendai virus reprogramming. Quality assessment the confirmed expression pluripotency markers, trilineage differentiation capacity and absence exogenous reprogramming factors. Both were genet...

OBJECTIVE Induced pluripotent stem cells are generated from somatic cells by direct reprogramming. These reprogrammed pluripotent cells have different applications in biomedical fields such as regenerative medicine. Although viral vectors are widely used for efficient reprogramming, they have limited applications in the clinic due to the risk for immunogenicity and insertional mutagenesis. Acco...

Malignancies are characterized by the reprogramming of epigenetic patterns. This reprogramming includes gains or losses in DNA methylation and disruption of normal patterns of covalent histone modifications, which are associated with changes in chromatin remodeling processes. This review will focus on the mechanisms underlying this reprogramming and, specifically, on the role of histone modific...

In this edition of COGEDE, we have invited colleagues to contribute their unique perspectives to illustrate the diverse ways that reprogramming is used in biology. This includes studying or harnessing reprogramming in various model systems as well as different experimental and natural contexts. Our goal is to demonstrate that reprogramming is not restricted to one stage of life, and it is not e...

Background: The understanding of nuclear reprogramming pathways provides important contributions to applied and basic sciences such as the development of autologous cellular therapies for the treatment of numerous diseases, the improved efficiency of animal-based biotechnology or the generation of functional gametes in vitro. Strategies such as nuclear transfer and induced reprogramming have be...

Induced pluripotent stem cells (iPSCs) have variable expression levels of a series of genes that affect their pluripotent potential, but the regulatory mechanisms controlling reprogramming remain unclear. By testing the efficiency of iPSC generation using Oct4, Sox2, Klf4 (termed OSK) plus one additional gene, we found that Rab32 improved reprogramming efficiency. We established a system for de...

During development, cell fate is specified precisely through programming by multiple complex elements and processes, including chromatin modifications that result in epigenetic marks. Once determined, cell fate is specified further only through maturation processes, which include differentiation and senescence. However, recent studies have shown that it is possible to influence cell fate throug...

Cellular reprogramming has been recently intensively studied experimentally. We developed a global potential landscape and kinetic path framework to explore a human stem cell developmental network composed of 52 genes. We uncovered the underlying landscape for the stem cell network with two basins of attractions representing stem and differentiated cell states, quantified and exhibited the high...