The intricate interactions between viruses and hosts include exploitation of host cells for viral replication by using many cellular resources, metabolites and energy. Tomato bushy stunt virus (TBSV), similar to other (+)RNA viruses, induces major changes in infected cells that lead to the formation of large replication compartments consisting of aggregated peroxisomal and ER membranes. Yet, it...

DNA replication initiates at chromosomal positions called replication origins. This review will focus on the activity, regulation and roles of replication origins in Saccharomyces cerevisiae. All eukaryotic cells, including S. cerevisiae, depend on the initiation (activity) of hundreds of replication origins during a single cell cycle for the duplication of their genomes. However, not all origi...

Although H5N1 influenza viruses have been responsible for hundreds of human infections, these avian influenza viruses have not fully adapted to the human host. The lack of sustained transmission in humans may be due, in part, to their avian-like receptor preference. Here, we have introduced receptor binding domain mutations within the hemagglutinin (HA) gene of two H5N1 viruses and evaluated ch...

Distributed Transactional Memory (DTM) is an attractive programming paradigm that aims at combining the simplicity of Transactional Memory (TM) with the scalability and failure resiliency achievable by exploiting the resource redundancy of distributed platforms. In these in-memory distributed platforms replication plays a role of paramount importance both for fault-tolerance and performance rea...

Recent studies suggest a possible takeover of host antimicrobial autophagy machinery by positive-stranded RNA viruses to facilitate their own replication. In the present study, we investigated the role of autophagy in coxsackievirus replication. Coxsackievirus B3 (CVB3), a picornavirus associated with viral myocarditis, causes pronounced intracellular membrane reorganization after infection. We...

Distributed storage systems support failures of individual devices by the use of replication or erasure correcting codes. While erasure correcting codes offer a better storage efficiency than replication for similar fault tolerance, they incur higher CPU consumption, higher network consumption and higher disk I/Os. To address these issues, codes specific to storage systems have been designed. T...

Stochastic simulations are able to capture the fine grain behaviour and randomness of outcome of biological networks not captured by deterministic techniques. As such they are becoming an increasingly important tool in the biological community. However, current efforts in the stochastic simulation of biological networks are hampered by two main problems: firstly the lack of complete knowledge o...

With the ever-growing amount of data, the network bandwidth requirement has increased greatly in Remote Backup System. The network bandwidth became the bottleneck of the whole system. The paper describes the Remote Backup System using Hash to identify and avoid sending duplicate data blocks between the Primary Node and the Secondary Node, thereby, to reduce the data replication network bandwidt...

Estimation of parametric variance functions for assays relies on transformation of standard deviations based on replication at each concentration. The quality of such estimates has been shown to have direct impact on the quality of inference based on the fitted calibration curve. The theory of Davidian and Carroll (1987) is used to demonstrate that ignoring unequal replication can lead to bias ...

In bacteria, several salvage responses to DNA replication arrest culminate in reassembly of the replisome on inactivated forks to resume replication. The PriA DNA helicase is a prominent trigger of this replication restart process, preceded in many cases by a repair and/or remodeling of the arrested fork, which can be performed by many specific proteins. The mechanisms that target these rescue ...