نتایج جستجو برای: recombinant protein production
تعداد نتایج: 1809349 فیلتر نتایج به سال:
background: light chain (lc) and heavy chain carboxyterminal subdomain (hcc) fragments are the most important parts of tetanus neurotoxin (tent) which play key roles in toxicity and binding of tent, respectively. in the present study, these two fragments were cloned and expressed in a prokaryotic system and their identity was confirmed using anti-tent specific polyclonal and monoclonal antibodi...
the manipulation of redox potential in secretory pathway by thiol reducing agents can be a strategy to improve the production levels of disulfide-bonded proteins including recombinant antibodies. here we have studied the influence of cysteamine on viability and the production level of igg4 in sp2.0 cells. for this purpose, the recombinant sp2.0 cells producing an anti cd33 igg4, were subjected ...
background: echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores,mainly dogs and foxes. the aim of this study is the production, purification and evaluation immunogenicity of new construction of eg95 protein. methods: the recombinant plasmid pet32-a+ used for eg95 expression was constructed...
Background: Increase of protein purity is a serious challenge in the production of recombinant therapeutic proteins. For this purpose, several strategies have been employed to purify the target protein, among which the affinity chromatography-based purification methods and tagged proteins such as Ni-NTA are common and but costly. Therefore column-free purification techniques, such as using elas...
Introduction: Plant ribosome inactivating proteins act as N-glycosidase enzyme and produce by several family of Caryophyllaceae such as Saponaria Officinalis. Different Isoforms of RIPs expressed by Saponaria Officinalis. SO6 isoform depurinate Adenine 4324 in the conserved GAGA loop of 28SrRNA and disrupts protein synthesis. The aim of this study was expression of SO6 isoform in E.coli and inv...
The manipulation of redox potential in secretory pathway by thiol reducing agents can be a strategy to improve the production levels of disulfide-bonded proteins including recombinant antibodies. Here we have studied the influence of cysteamine on viability and the production level of IgG4 in Sp2.0 cells. For this purpose, the recombinant Sp2.0 cells producing an anti CD33 IgG4, were subjected ...
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