Gene and whole-genome duplications are widespread in plant nuclear genomes, resulting in sequence heterogeneity. Identification of duplicated genes may be particularly challenging in highly redundant genomes, especially when there are no diploid parents as a reference. Here, we developed a pipeline to detect the different copies in the ribosomal RNA gene family in the hexaploid grass Spartina m...

avian intestinal spirochetosis (ais) is a disease of birdscharacterized by a marked colonization of the cecum and/or rectumwith anaerobic intestinal bacteria of the genus brachyspira. thepurpose of the study was to determine the occurrence of brachyspiraspp from avian sources in iran and to characterize selected isolates bybiochemical and molecular methods. out of 165 fecal samples obtained fro...

BACKGROUND Ribosomal RNAs have been widely used for identification and classification of species, and have produced data giving new insights into phylogenetic relationships. Recently, multilocus genotyping and even whole genome sequencing-based technologies have been adopted in ambitious comparative biology studies. However, such technologies are still far from routine-use in species classifica...

Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates...

BACKGROUND 16S ribosomal DNA (rDNA) amplicon sequencing is frequently used to analyse the structure of bacterial communities from oceans to the human microbiota. However, computational power is still a major bottleneck in the analysis of continuously enlarging metagenomic data sets. Analysis is further complicated by the technical complexity of current bioinformatics tools. RESULTS Here we pr...

Abstract Background The two-spotted spider mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), is one of the most damaging mites in agriculture. Due to concern for intensive use synthetic acaricides, entomopathogenic fungi represents a feasible alternative T. management. In present study, 7 isolates Metarhizium were characterized physiological and molecularly (based on ITS1-5.8s-ITS...

While mammary gland tissues (MGTs) are difficult to sample without risks for cow's health or milk production, analysis used in routine assess dairy cow udder's health. This study aimed identify, quantify, compare the and MGTs microbiota of macroscopically healthy bovine glands (MG) order evaluate their degree similarity. We harvested 13 couples samples, originated from same quarter at culling. ...

Here we present the Cytoscape app version of our association network inference tool CoNet. Though CoNet was developed with microbial community data from sequencing experiments in mind, it is designed to be generic and can detect associations in any data set where biological entities (such as genes, metabolites or species) have been observed repeatedly. The CoNet app supports Cytoscape 2.x and 3...