نتایج جستجو برای: puc18
تعداد نتایج: 196 فیلتر نتایج به سال:
cDNA microarray is an established technique. However, difficulties such as handling tissue samples under RNase-free conditions, the heterogeneous tumor composition, i.e. non-malignant versus malignant cells and different pathologic types of malignant cells, and lack of appropriate reference may limit the potentially benefit of this method in clinical use. In this study, we examined how standard...
Hydroxychavicol (HC; 10 - 50 microM), a betel leaf component, was found to suppress the 2% H(2)O(2)-induced lucigenin chemiluminescence for 53 - 75%. HC (0.02 - 2 microM) was also able to trap superoxide radicals generated by a xanthine/xanthine oxidase system with 38 - 94% of inhibition. Hydroxyl radicals-induced PUC18 plasmid DNA breaks was prevented by HC (1.6 - 16 microM). A 24-h exposure o...
The complete nucleotide sequence of pRGO1, a cryptic plasmid from Propionibacterium acidipropionici E214, was determined. pRGO1 is 6, 868 bp long, and its G+C content is 65.0%. Frame analysis of the sequence revealed six open reading frames, which were designated Orf1 to Orf6. The deduced amino acid sequences of Orf1 and Orf2 showed extensive similarities to an initiator of plasmid replication,...
GEArray TM analysis The non-radioactive GEArray TM original and Q series cDNA expression array filters (Original Series Mouse Common Cytokine-1 Gene Array, Cat. No.: mGEA101207N and Mouse Inflammatory Cytokine/Receptor Gene Array Q series, Cat. No.: MM-002N-4) were used, and hybridization procedures were as described by the manufacturer (SuperArray Inc., Bethesda, MD). Total RNA was isolated fr...
زمینه و هدف: سندرم بوتولیسم توسط نوروتوکسین باکتری کلستریدیوم بوتولینوم ایجاد می شود. این نوروتوکسین دارای 7 سروتیپ از a-g می باشد. بهترین راه برای جلوگیری از سندرم بوتولیسم ایجادشده توسط bont/b، استفاده از واکسن های نوترکیب ساخته شده از ناحیه اتصالی آن (به علت داشتن اپی توپ های کافی برای تحریک سیستم ایمنی) است. در این پژوهش بیان زیرواحد اتصالی نوروتوکسین بوتولینوم تیپ b، به منظور کاندید واکسن ...
مقدمه: بیوپلاستیک پلی هیدروکسی بوتیرات کاربرد گسترده در صنایع مختلف و ساخت تجهیزات پزشکی دارد. هزینه های استخراج و تخلیص پلی هیدروکسی بوتیرات در اشریشیا کولی، بخش اعظم هزینه های تولید را به خود اختصاص داده است. ژن e باکتریوفاژ фx174 با ایجاد تونل غشایی برای لیز باکتری میزبان استفاده می شود. هدف از انجام این مطالعه، بررسی عملکرد ژن e در ایجاد تونل غشایی و رهاسازی پلی هیدروکسی بوتیرات تولیدشده در...
interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...
we have two part in this thesis, at first: the interaction of native calf thymus dna (ct-dna) with two anthraquinones including quinizarin (1,4- dihydroxy anthraquinone) and danthron (1,8- dihydroxy anthraquinone) in a mixture of 0.04 m brittone-robinson buffer and 50% of ethanol were studied at physiological ph by uv-vis absorption, florescence, circular dichroism spectroscopic methods, viscos...
A 5-kbp region upstream of the are-ben-cat genes was cloned from Acinetobacter sp. strain ADP1, extending the supraoperonic cluster of catabolic genes to 30 kbp. Four open reading frames, salA, salR, salE, and salD, were identified from the nucleotide sequence. Reverse transcription-PCR studies suggested that these open reading frames are organized into two convergent transcription units, salAR...
Candida species are opportunistic pathogens that infect immunocompromised and/or immunosuppressed patients, particularly in hospital facilities, that besides representing a significant threat to health increase the risk of mortality. Apart from echinocandins and triazoles, which are well tolerated, most of the antifungal drugs used for candidiasis treatment can cause side effects and lead to th...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید