The original limited proteolysis approach was proposed for large (multidomain) proteins with post-translational modifications for obtaining of their novel fragments. It was realized for human immunoglobulins representing two subclasses IgG2 and IgG3. This approach was based on two techniques: masking of protein regions which are normally susceptible to proteolytic enzymes and increasing the pos...

Kupres cheese is a traditional Bosnian full-fat hard cheese. This paper deals with the change of protein and fatty acid profiles, mineral content antioxidant potential during period three months its ripening. Proteolysis was monitored by electrophoresis total extracts water-soluble fractions under reducing conditions, whereas profiles were determined gas chromatography. The in ripened cheeses u...

Proteolysis of the amyloid precursor protein (APP) liberates various fragments including the proposed initiator of Alzheimer disease-associated dysfunctions, amyloid-β. However, recent evidence suggests that the accepted view of APP proteolysis by the canonical α-, β-, and γ-secretases is simplistic, with the discovery of a number of novel APP secretases (including δ- and η-secretases, alternat...

Actomyosin was prepared from human myocardium and its protein composition was examined by SDS-polyacrylamide gel electrophoresis. For some preparations, particularly actomyosin isolated from elderly subjects, a high molecular weight (HMW) band (identified as a breakdown product of myosin heavy chain) appeared, while the troponin-T subunit decreased. Myofibril associated protease (MFP) activity ...

In the histone code hypothesis histone proteolysis is depicted as a novel kind of irreversible post-translational modifications of histones. Site specific proteolysis of histone is hypothesized as a process for removal of epigenetic signature of histones. However, though there are many reports depicting histone proteolysis, there are very few reports on characterization of a histone protease. I...

The 26S proteasome is a multisubunit complex that catalyzes ATP-dependent proteolysis of cellular proteins. It eliminates misfolded proteins, as well as labile regulatory proteins, thereby serving a central role in maintaining cellular homeostasis. The bulk of the known substrates of the 26S proteasome are earmarked for proteolysis by covalent modification with a multiubiquitin chain, which is ...

Four decades of proteasome research have yielded extensive information on ubiquitin-dependent proteolysis. The archetype proteasomes is a 20S barrel-shaped complex that does not rely ubiquitin as degradation signal but can degrade substrates with considerable unstructured stretch. Since roughly half all in most eukaryotic cells are free complexes, ubiquitin-independent protein may coexist by th...

Abstract Proteolysis is the rate-limiting step in mineralization of organic nitrogen into ammonium (NH 4 + ) and thereby ammonia 3 released during composting. However, dynamics bacterial proteolytic communities related to NH emissions composting systems are mostly unknown. This study aimed examine compare effects hyperthermophilic pretreatment (HPC) traditional (TC) methods on (i) difference lo...

The hypothesis that increased ADAMTS13 (a disintegrin and metalloprotease with thrombospondin repeats) activity or increased susceptibility of von Willebrand factor (VWF) to proteolysis by ADAMTS13 may underlie type I von Willebrand disease (VWD) in some patients was investigated. Plasma from 4 patients with type I VWD was cryoprecipitated. ADAMTS13 activity in the VWF-poor cryodepleted fractio...

Acute kidney injury (AKI) is associated with mitochondrial fragmentation, which contributes to mitochondrial damage and tubular cell apoptosis. Mitochondrial fragmentation involves the cleavage of both mitochondrial outer and inner membranes. Cleavage of the outer membrane results from Drp-1-mediated fission activation and Bak-promoted fusion arrest, but the molecular mechanism of inner membran...