نتایج جستجو برای: polyhydroxybutyrate phb

تعداد نتایج: 1668  

2014
Amanda Bernardi Cláudia Gai Jingnan Lu Christopher Brigham Anthony Sinskey

Background Ralstonia eutropha is bacterium known to naturally produce polyhydroxybutyrate (PHB) as carbon storage during nutrient starvation. Previously studies [1] showed that it is possible through the incorporation of an engineered biosynthetic pathway, to redirect carbon flux from PHB to the production of Isobutanol (IBT), a biofuel largely studied to replace the current fossil fuels in exi...

2008
David Leonard Nicholas D. Lindley

Centre de Bioingenierie Gilbert Durand, UMR CNRYINSA and Lab. Ass. INRA, lnstitut National des Sciences Appl iq uCes, Complexe Scientifique de Rangueil, 31077 Toulouse cedex 4, France The growth behaviour of AIcaligenes eutrophus on phenol was investigated in continuous cultures to identify the phenomena limiting both growth efficiency and substrate degradation rates. It was shown that the fixe...

Journal: :Biochemistry 2001
Y Jia W Yuan J Wodzinska C Park A J Sinskey J Stubbe

The Class I and III polyhydroxybutyrate (PHB) synthases from Ralstonia eutropha and Chromatium vinosum, respectively, catalyze the polymerization of beta-hydroxybutyryl-coenzyme A (HBCoA) to generate PHB. These synthases have different molecular weights, subunit composition, and kinetic properties. Recent studies with the C. vinosum synthase suggested that it is structurally homologous to bacte...

2017
Sandrine Straub Philipp E. Hirsch Patricia Burkhardt-Holm

Research on the uptake and effects of bioplastics by aquatic organisms is still in its infancy. Here, we aim to advance the field by comparing uptake and effects of microplastic particles (MPP) of a biodegradable bioMPP (polyhydroxybutyrate (PHB)) and petroleum-based MPP (polymethylmethacrylate (PMMA)) in the freshwater amphipod Gammarus fossarum. Ingestion of both MPP in different particle siz...

2012
Mingji Li Junshu Wang Yanping Geng Yikui Li Qian Wang Quanfeng Liang Qingsheng Qi

BACKGROUND For metabolic engineering, many rate-limiting steps may exist in the pathways of accumulating the target metabolites. Increasing copy number of the desired genes in these pathways is a general method to solve the problem, for example, the employment of the multi-copy plasmid-based expression system. However, this method may bring genetic instability, structural instability and metabo...

2014
Wei Zhang Ruben Shrestha Rachael M. Buckley Jamie Jewell Stefan H. Bossmann JoAnne Stubbe Ping Li

Polyhydroxybutyrate (PHB) synthases catalyze the polymerization of 3-(R)-hydroxybutyrate coenzyme A (HBCoA) to produce polyoxoesters of 1-2 MDa. A substrate analogue HBCH2CoA, in which the S in HBCoA is replaced with a CH2 group, was synthesized in 13 steps using a chemoenzymatic approach in a 7.5% overall yield. Kinetic studies reveal it is a competitive inhibitor of a class I and a class III ...

2010
Thore Rohwerder Roland H Müller

Nowadays a growing demand for green chemicals and cleantech solutions is motivating the industry to strive for biobased building blocks. We have identified the tertiary carbon atom-containing 2-hydroxyisobutyric acid (2-HIBA) as an interesting building block for polymer synthesis. Starting from this carboxylic acid, practically all compounds possessing the isobutane structure are accessible by ...

Journal: :Journal of colloid and interface science 2016
María José Fabra Amparo López-Rubio Luis Cabedo Jose M Lagaron

This work compares the effect of adding different biopolyester electrospun coatings made of polycaprolactone (PCL), polylactic acid (PLA) and polyhydroxybutyrate (PHB) on oxygen and water vapour barrier properties of a thermoplastic corn starch (TPCS) film. The morphology of the developed multilayer structures was also examined by Scanning Electron Microscopy (SEM). Results showed a positive li...

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