There is broad species variation in the type of cAMP-dependent protein kinase isozyme present in supernatant fractions of heart homogenates as determined by DEAE-cellulose chromatography, Isozyme I, which elutes at less than 0.1 M NaCl, is predominant in mouse and rat hearts; while isozyme II, which elutes at greater than 0.1 M NaCl, is the predominant type in beef and guinea pig. Human and rab...

Extracts from preparations of partially purified Anaplasma marginale revealed low levels of lactate dehydrogenase (LDH). Enzyme inhibition by immune sera further indicated that A. marginale possesses a protein moiety the same as that of the normal red blood cell (RBC), although data suggested an alteration of LDH(1) from that observed in normal RBC. Bimodal isozyme distribution was detected aft...

In the present study six different populations of L. orbonalis were collected and subjected to analysis of genetic variability in terms of carboxylesterase isozyme pattern and DNA polymorphism using RAPD-PCR. Pattern of carboxylesterase revealed a similar isozyme cluster in the populations namely, sivaganga (population-3), dindigal (population-4), virudhunagar (population-5) and coimbatore (pop...

flECENT INVESTIGATIONS by Markert and Moeller1 and Wieland and 1 Pfieiderer2 have shown by electrophoresis that lactic dehydrogenase ( LDH ) occurs in multiple molecular forms, with certain molecular species of the enzyme being present in greater amount in certain tissues. The electrophoretic form which we call isozyme I ( see fig. 1 ) predominates in extracts of heart muscle while the isozyme ...

Human platelets and bovine lens fibers contain lactate dehydrogenase-3 (LDH-3) as the predominant isozyme but only very faint traces of LDH-5. Since the platelets and lens fibers, like mature human erythrocytes, lack a nucleus, the results strengthen the case for a previously developed association between LDH-5 and the cell nucleus. These three cell types are mainly anaerobic, and therefore the...

Glucosamine 6-phosphate synthase (EC 5.3.1.19) purified from various rat tissues by a procedure involving chromatography on diethylaminoethyl Sephadex and hydroxylapatite were characterized by means of isoelectric focusing. The non-hepatic isozyme, previously reported to be present in Yoshida sarcoma, has a pI of 4.1 and is distinguished from the hepatic isozyme, with a pI of 5.0. The pI 4.1 fo...