OBJECTIVES The aim of this paper is to attribute Panton-Valentine leucocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) to clonal lineages by molecular typing with special reference to isolates exhibiting spa type t008/multilocus sequence type (MLST) ST8 [widely disseminated in the USA as 'community-associated MRSA (caMRSA) USA300']. METHODS PVL-positive MRSA (n = 117) ...

Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to investigate ongoing transmission of leprosy in regions of endemicity. To enable wider implementation of these molecular analyses, novel real-time PCR-high-resolution melt (RT-PCR-HRM) assays without allele-specific primers or probes and post-PCR sample handling were developed. For the detection of mutations ...

Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species of organisms, no typing method is available; for others, typing methods are tedious. We developed a method of amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce strain-specific electrophoretic patterns from c...

PURPOSE To characterize methicillin-resistant Staphylococcus aureus (MRSA) strains by molecular typing based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of spa gene and to assess the utility of spa genotyping over bacteriophage typing in the discrimination of the strains. MATERIALS AND METHODS Studies were undertaken on 125 MRSA strains representing the mo...

Leptospirosis is a worldwide widespread zoonosis caused by Leptospira genus. We report an acute leptospirosis case in puppy housed at municipal kennel and the subsequent diagnostic investigations carried out on all dogs kennel. Laboratory investigation included mainly microagglutination test, real-time PCR, multi-locus sequence typing (MLST) for Other agents of infection were excluded. The resu...

The traditional cultural method (PCR and Real-Time PCR) for Salmonella spp. detection identification is laborious time-consuming. A qualitative LAMP detecting was validated in compliance with ISO 16140:2016. results show a relative accuracy, sensitivity, specificity of 100% comparison the reference 6579-1:2017; LOD50 set as 0.4 CFU/g. Additionally, field study carried out comparing kit, commerc...