نتایج جستجو برای: nucleic acid sequence based amplification
تعداد نتایج: 3885413 فیلتر نتایج به سال:
Infectious disease diagnosis in point-of-care settings can be greatly improved through integrated, automated nucleic acid testing devices. We have developed an early prototype for a low-cost system which executes isothermal DNA amplification coupled to nucleic acid lateral flow (NALF) detection in a mesofluidic cartridge attached to a portable instrument. Fluid handling inside the cartridge is ...
INTRODUCTION Genital Chlamydia trachomatis infection is the leading cause of bacterial sexually transmitted diseases in industrial countries, particularly among young people. The consequences of chlamydial infections may involve pelvic inflammatory disease, ectopic pregnancy and tubal factor infertility. METHODS Available tests for detection of chlamydia in men and women include culture in ti...
enteroviruses are the causative agents of a number of diseases in humans. group b coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. genomic data of enteroviruses has allowed developing new molecular approaches such as nucleic acid sequence based amplification (nasba) for detection of such viruses. in this study, coxsackievirus b3 (cvb3) was de...
A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based o...
Nucleic acid testing for infectious diseases at the point of care is beginning to enter clinical practice in developed and developing countries; especially for applications requiring fast turnaround times, and in settings where a centralized laboratory approach faces limitations. Current systems for clinical diagnostic applications are mainly PCR-based, can only be used in hospitals, and are st...
The more than three decades of research in nucleic acid nanotechnology has led to the thrilling progress rationally designed structures and artificial molecular devices with programmable functions various applications. Nucleic acid–based aggregates feature precise recognition sequence programmability, versatility, as well marked biocompatibility, providing promising candidates for biomedical In...
BACKGROUND Development of a practical gene point-of-care testing device (g-POCT device) requires innovative detection methods for demonstrating the results of the gene amplification reaction without the use of expensive equipment. We have studied a new method for the sequence-specific visual detection of minute amounts of nucleic acids using precipitation reaction by addition of cationic polyme...
Background: Salmonella enterica is a zoonotic pathogen of substantial concern to human and animal health leading cause morbidity mortality in people worldwide. Loop-mediated isothermal amplification (LAMP) technology new type nucleic acid technology, which has the characteristics high specificity, sensitivity, simple operation, convenience, low cost. This study aims establish rapid detection me...
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