نتایج جستجو برای: ns1

تعداد نتایج: 2089  

2016
Jianping Wu Chee-Keng Mok Vincent Tak Kwong Chow Y. Adam Yuan Yee-Joo Tan

We have previously shown that a non-structural protein 1 (NS1)-binding monoclonal antibody, termed as 2H6, can significantly reduce influenza A virus (IAV) replication when expressed intracellularly. In this study, we further showed that 2H6 binds stronger to the NS1 of H5N1 than A/Puerto Rico/8/1934(H1N1) because of an amino acid difference at residue 48. A crystal structure of 2H6 fragment an...

Journal: :The Southeast Asian journal of tropical medicine and public health 2011
Anita Chakravarti Amod Kumar Sonia Malik

We analyzed the utility of a commercial NS1 antigen based ELISA (Panbio Dengue Early ELISA) for detection of dengue infection during the early acute phase and anti-Dengue IgM capture ELISA for detecting dengue infection in patients in dengue endemic settings. A total of 145 serum samples collected from febrile suspected dengue patients were tested for the presence of anti-dengue IgM antibody us...

Journal: :Nucleic acids research 1999
A M Falcón P Fortes R M Marión A Beloso J Ortín

A screening for human proteins capable of interacting with influenza virus NS1 has been carried out using the two-hybrid genetic trap in yeast. A cDNA corresponding to the human homologue of Drosophila melanogaster Staufen protein (hStaufen) was isolated that fulfilled all genetic controls of the two-hybrid protocol. Using a hStaufen cDNA isolated from a lambda human library, the interaction of...

Journal: :The Journal of infectious diseases 2010
Hongbo Zhou Jiping Zhu Jiagang Tu Wei Zou Yong Hu Zhengjun Yu Wensi Yin Yongtao Li Anding Zhang Yurong Wu Ziniu Yu Huanchun Chen Meilin Jin

To study the effect of NS1 eIF4GI binding domain on virulence and pathogenicity of H5N1 influenza A virus, 5 recombinant H5N1 viruses encoding eIF4GI binding domain-truncated NS1 proteins and parental NS1 (NS1‐wt) were generated by an 8‐plasmid-based reverse genetics system. The results indicated that the recombinants with the addition of 5‐amino acid and the deletion position of 85-89 in NS1‐w...

Journal: :The Journal of general virology 1999
M N Fleeton B J Sheahan E A Gould G J Atkins P Liljeström

Recombinant Semliki Forest virus (rSFV) vaccines encoding louping ill virus (LIV) genes prME and NS1 were examined. Cells transfected with rSFV-prME RNA showed correct processing of the precursor prME and the release into the medium of M and E proteins in particulate form, whilst rSFV-NS1-transfected cells secreted glycosylated, heat-labile NS1 dimers. Mice immunized with rSFV particles produce...

2011
Laura Bavagnoli William G. Dundon Anna Garbelli Bianca Zecchin Adelaide Milani Geetha Parakkal Fausto Baldanti Stefania Paolucci Romain Volmer Yizeng Tu Chuanyue Wu Ilaria Capua Giovanni Maga

The Non-structural 1 (NS1) protein of avian influenza (AI) viruses is important for pathogenicity. Here, we identify a previously unrecognized tandem PDZ-ligand (TPL) domain in the extreme carboxy terminus of NS1 proteins from a subset of globally circulating AI viruses. By using protein arrays we have identified several human PDZ-cellular ligands of this novel domain, one of which is the RIL p...

Journal: :Clinical and vaccine immunology : CVI 2012
Alvina Clara Felix Camila Malta Romano Cristiane de Campos Centrone Célia Lima Rodrigues Lucy Villas-Boas Evaldo Stanislau Araújo Andréia Manso de Matos Karina Inácio Carvalho Celina Maria Turchi Martelli Esper Georges Kallas Claúdio Sérgio Pannuti José Eduardo Levi

In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary inf...

Journal: :Journal of virology 2004
Randall J Owens Chang Limn Polly Roy

The insect-borne Bluetongue virus (BTV) is considered the prototypic Orbivirus, a member of the Reovirus family. One of the hallmarks of Orbivirus infection is the production of large numbers of intracellular tubular structures of unknown function. For BTV these structures are formed as the polymerization product of a single 64-kDa nonstructural protein, NS1, encoded by the viral double-strande...

2014
Xi-Xia Ding Xiao-Feng Li Yong-Qiang Deng Yong-Hui Guo Wei Hao Xiao-Yan Che Cheng-Feng Qin Ning Fu Xi Zhou

The early diagnosis of West Nile virus (WNV) infection is important for successful clinical management and epidemiological control. The non-structural protein 1 (NS1) of flavivirus, a highly conserved and secreted glycoprotein, is abundant in the serum of flavivirus-infected patients and represents a useful early diagnostic marker. We developed a WNV-specific NS1 antigen-capture ELISA using two...

Journal: :PLoS Neglected Tropical Diseases 2008
Philippe Dussart Laure Petit Bhety Labeau Laetitia Bremand Alexandre Leduc David Moua Séverine Matheus Laurence Baril

BACKGROUND We compared the performance of two new commercial tests for the detection of dengue NS1 protein during the clinical phase of dengue virus (DENV) infection-an immunochromatographic test allowing rapid detection of the NS1 antigen, Dengue NS1 Ag STRIP (Bio-Rad Laboratories - Marnes La Coquette, France), and a two-step sandwich-format microplate enzyme-linked immunosorbent assay (ELISA)...

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