From the Department of Ophthalmology, Biochemistry Research Laboratory (The Sir Isaac and Lady Wolfson Ophthalmic Research Laboratories), Hebrew University-Hada.ssah Medical School, Jerusalem, Israel. 1 Present address: Department of Biophysics, Weizmann InstituteofScience,Rehovot,Israel. Nonstandard abbreviations used: MPS, mucopolysaccharides (glycosaminoglycans),mucopolysaccharidoses (with r...

Oral Cleansing, Spiders, Gnathocoxal Gland, Histochemistry, Micromorphological Investigations Histochemical and micromorphological investigations of the gnathocoxal gland in spiders brought out its uniquely adaptive cleansing function. The prey particulates, caught in the oral mi­ crostructures, are bound with mucopolysaccharides or glyco-muco-proteins from the glandular se­ cretions in Araneus...

An enzymatic reaction has been identified in the isthmus of hen oviduct in which sulfate is transferred from 3’-phosphoadenosine 5’-phosphosulfate to mucopolysaccharide acceptors (1). From studies of the reaction with monosaccharides and oligosaccharides as model acceptors (‘2) it was concluded that one or more enzymes in the preparations employed catalyze a direct transfer of sulfate from PAPS...

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

Umbilical cord was shown to contain chondroitin sulfate B and a small amount of glucosamine-containing sulfated mucopolysaccharide, in addition to hyaluronic acid and chondroitin sulfate C. All of the hyaluronic acid and part of the chondroitin sulfate C could be extracted directly with dilute sodium chloride; the rest of the chondroitin sulfate C and the chondroitin sulfate B were released onl...