Abstract The complex nature of the immune system requires deep interrogation at single-cell level. Flow cytometry using CyTOF® technology employs antibodies tagged with isotopically enriched metals instead fluorophores, resulting in discrete signals that facilitate highly multiparametric characterization on a CyTOF enables analysis over 50 parameters simultaneously without impediments spectral ...

Background: The Seshachalam hill ranges of Eastern Ghats are known for their endemic flora. There were seven species situated here in which present plant Terminalia pallida (T. pallida) is one, this Endemic semi-tree has a great medicinal value; at the same time, due to presence different types secondary metabolites, they easily form nanoparticles and exhibit certain high-quality ...

The aim of this work is to study standardization parameters and pharmacological properties Harpagophytum procumbens (H. procumbens) root dry extract.
 Materials methods – obtaining H. extract was performed in accordance with developed scheme that characterized by simplicity cost-effectiveness technology compared analogous scheme. Study obtained the requirements monograph «Harpagophyti extr...

Pollution in the environment due to accumulation of potentially toxic metals results deterioration soil and water quality, thus impacting health all living organisms including microbes. In present investigation, a functional metagenomics approach was adopted mine genes involved metal tolerance from contaminated site. Eukaryotic cDNA library (1.0–4.0 kb) screened for providing cadmium (Cd) toxic...

Andrographis paniculata (AP) is an ancient herb known for its medicinal and therapeutic values. In this study, we aimed to evaluate the antibacterial activity cytotoxicity of AP herbal mouthwash. Aqueous extract was used prepare The product tested against selected oral pathogens namely Actinomyeces viscosus, Staphylococcus aureus, Streptococcus mutans, sobrinus, Porphyromonas gingivalis using a...

Metals have been mined and used since ancient times. The industrial era has seen a sharp increase in both the amounts and variety of metals that find applications in industry. The inadvertent release of metals, such as from fossil fuel consumption, also adds to the global burden. A number of catastrophic outbreaks have alerted us to the occupational and environmental health risks. Life on this ...

a multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (sf) of irradiated cells. the estimation of sf based on the cell‑growth curve information is the major advantage of this assay. in this study, the utility of multiple‑mts assay for the sf estimation of irradiated ht‑29 colon cancer cells, which were plated before irradiation, w...

Background: Cadmium is one of the most toxic heavy metals in our environment having a very strong ability to accumulate in body organs, especially in kidney. The present study was done to determine the genotoxicity and cytotoxicity in kidneys of rats exposed to cadmium. Methods: Male rats (n=30), kept in standard conditions were used in this study. The animals were randomly divided into 2 group...

To reveal the impact of Non-Flooding controlled Irrigation (NFI) on the bioavailability and bioaccumulation of metals (Cu, Pb, Cd and Cr) in rice fields, metals concentration in different organs of rice plant growing under both Flooding Irrigation (FI) and NFI were measured. It indicated that metals concentrations in root are always the highest one among all the plant organs and in the spike is...

A set of nine recombinant heavy metal-specific luminescent bacterial sensors belonging to Gram-negative (Escherichia and Pseudomonas) and Gram-positive (Staphylococcus and Bacillus) genera and containing various types of recombinant metalresponse genetic elements was characterized for heavy metal bioavailability studies. All nine strains were induced by Hg and Cd and five strains also by Zn. As...