In the present paper we explored the capacity of yeast Saccharomyces cerevisiae as host for heterologous expression of human Aquaporin-1. Aquaporin-1 cDNA was expressed from a galactose inducible promoter situated on a plasmid with an adjustable copy number. Human Aquaporin-1 was C-terminally tagged with yeast enhanced GFP for quantification of functional expression, determination of sub-cellul...

Yeast cell biologists use a variety of fluorescent protein tags for determining protein localization and for measuring protein dynamics using fluorescence recovery after photobleaching (FRAP). Although many modern fluorescent proteins, such as those with photoactivatable and photoconvertible characteristics, have been developed, none has been exploited for studies in budding yeast. We describe ...

W42 Effect of a live-yeast-based product on colostrum quality and milk yield in first month of lactation on a private dairy farm. C. Julien*1,2, A. Fernandez3, and J. P. Marden3, 1INRA, UMR1289 TANDEM, Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, Castanet-Tolosan, France, 2Université de Toulouse, INPT ENSAT, INP-ENVT, UMR1289 TANDEM, Castanet-Tolosan, France, 3Lesaffre Feed Add...

Microbes typically live in communities. The spatial organization of cells within a community is believed to impact the survival and function of the community(1). Optical sectioning techniques, including confocal and two-photon microscopy, have proven useful for observing spatial organization of bacterial and archaeal communities(2,3). A combination of confocal imaging and physical sectioning of...

Ninety-six crossbred sows and gilts were used to study the effect of yeast culture (Diamond V. Mills, Cedar Rapids, IA) on apparent nutrient digestibilities and reproductive performance from d 60 of gestation through d 21 of lactation. Sows were allotted to treatment groups based on parity and BW. Four dietary treatments were made by substituting 0, .5, 1.0, or 2.0% yeast culture for wheat midd...

The purpose of this review article is to discuss the role probiotics in animal nutrition and health. In last 15 years, have become increasingly popular many production systems. Inadequate scientifically-based, all-encompassing, unified data on effects monogastric ruminant animals prompted current review. Feed supplements containing live microorganisms, known as probiotics, are shown improve int...

The purpose of this work was to study the effect of initial pH and yeast Saccharomyces Cerevisiae on biogas production from tofu wastewater (TW). The initial pH was varied in ranging of 5 – 9 in substrate without yeast (T5-T9) and with yeast (TY5-TY9). The results showed that optimum initial pH was 8. The maximum biogas was resulted in T8 (275 mL) and TY8 (421 mL). Yeast addition increased tota...

Yeast has been a very helpful organism for centuries, especially with respect to fermentation of sugars and production of bread. However, for an even longer time, yeast has been a distant relative of humans having diverged from a common ancestor, about one billion years ago. More than one third of the yeast genes have human counterparts, despite this evolutionary distance. Yeast and human ortho...