We established sterile clones of a medicinal plant dragonhead Dracocephalum charkeviczii Prob. (Lamiaceae). used shoots the plants from natural habitat, notably leaf nodes with axillary bud, as explants, sterilized explants in 0.1 % Diocide for 4 minutes and cultivated them on agarized nutrient half-strength MS medium vitamins, 2 sucrose, 0.4 agar, 1 mg/l indolylbutyric acid (IBA), or 0.5 1.0 6...

This study was carried out to determine in vitro development using Black cumin leaf and stem explants. ?ameli black variety used as plant material. Five different nutrient mediums (1. LS2.5, 2. MS, 3. MS + 0.5 mg.l-1 IAA, 4. BAP, 5. IAA BAP) containing 30 g sugar were this study. As a result of the research, 100% callus formation detected explants cultured number 1 5 mediums. These followed by ...

Wild plant species are of great interest as a source pharmacologically valuable compounds but number them endemic and/or endangered ones. Modern biotechnology can provide reliable methods for their utilization without disturbing natural populations. In vitro culture Rhodiola being intensively developed to include into various biotechnological programmes. Aim. Development protocol direct rosea L...

In present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic emb...

Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25-30 days of infection. Optim...