A particulate enzyme system from Micrococcus roseus R2J has been obtained which catalyzes the biosynthesis of a peptidoglycan. The preparation catalyzes the incorporation of L-threonine onto the e-amino group of lysine in the presence of transfer RNA @RNA), supernatant solution, and ATP. Crude l%-L-threonyl-tRNA will substitute for these additional requirements. The threonyl-tRNAs of this organ...

The study was focused on the effect of drought and waterlogging stresses in two-year pot experiments peat substrate content glucose, fructose sucrose free amino acids potato tubers four cultivars (yellow-fleshed Laura, Marabel, Milva blue-fleshed Valfi) after 71 days exposure to conditions (BBCH 909). Drought increased levels fructose, three except for cv. Laura. stress l-proline (+248.4%), l-h...

OBJECTIVE A study was conducted to test six threonine (Thr) levels (0.39%, 0.44%, 0.49%, 0.54%, 0.59%, and 0.64%) to estimate the optimal dietary Thr requirements for Longyan laying ducks from 17 to 45 wk of age. METHODS Nine hundred Longyan ducks aged 17 wk were assigned randomly to the six dietary treatments, where each treatment comprised six replicate pens with 25 ducks per pen. RESULTS...

Genetic breeding of microbial amino acid producers has been done conventionally by acquiring auxotrophic mutants or regulatory mutants based on fundamental knowledge of amino acid biosynthetic pathways and their regulatory mechanisms. The conventional techniques are effective in that a number of amino acids, such as lglutamate, L-lysine, L-phenylalanine, L-threonine, larginine, L-isoleucine, L-...

A particulate enzyme system from Micrococcus roseus R2J has been obtained which catalyzes the biosynthesis of a peptidoglycan. The preparation catalyzes the incorporation of L-threonine onto the e-amino group of lysine in the presence of transfer RNA @RNA), supernatant solution, and ATP. Crude l%-L-threonyl-tRNA will substitute for these additional requirements. The threonyl-tRNAs of this organ...

When oxidized to cysteic acid by performic acid or converted to carboxymethylcysteine by alkylation of the reduced enzyme with iodoacetate, a total of six half-cystine residues/subunit are found in L-threonine dehydrogenase (L-threonine: NAD+ oxidoreductase, EC 1.1.1.103; L-threonine + NAD(+)----2-amino-3-oxobutyrate + NADH) from Escherichia coli K-12. Of this total, two exist in disulfide link...