The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethylene glycol in different solutions and the time of addition to the cell culture medium were critic...

We describe a case of the isolation of Bartonella quintana from the parotid gland of an apparently healthy man. Pathological examination showed intraparotid granulomatous abscessual lymphadenitis. Diagnosis was made on the basis of high titers of immunoglobulin G (IgG) and IgM antibodies and of culture isolation of a causative agent from parotid aspirate.

the operating room. Non-enzymatically isolated stromal vascular fraction contained cells expressing CD44, CD73, CD90 and CD105 that when expanded in culture differentiated along adipogenic and osteogenic lineages. When compared to enzymatic isolation, mechanical isolation methods required less time but produced lower cell yields. Two articles reported improved volume retention in fat grafts sup...

This paper reports the first isolation of a spotted fever group rickettsia from an Amblyomma cooperi ixodid collected from a capybara (Hydrochaeris hydrochaeris) in an endemic area of spotted fever in the County of Pedreira, State of São Paulo, Brazil. Isolation was performed in Vero cell culture and submitted to immunofluorescence, using antibody from Rickettsia rickettsii-positive human serum.

OBJECTIVES Primary cell cultures derived from the corpus cavernosum are frequently used as in vitro models to define cellular mechanisms involved in erectile function. However, previous studies often lack detailed isolation protocols or a precise characterisation of the culture composition excluding especially contaminating fibroblasts. This study aimed at critically analysing and reproducing r...

The isolation of beta haemolytic streptococci was compared using Columbia sheep blood agar with and without gentamicin in a final concentration of 5.5 micrograms per ml. The inclusion of gentamicin gave greatly improved results whenever the isolation of this organism in pure culture was complicated by overgrowth with Gram-negative bacilli or staphylococci.

Exosomes, (50-150 nm sized vesicles), are secreted by all cells and found in all body fluids investigated. Isolation and characterization of exosomes from cell culture systems and body fluids provide valuable information about the biological system. The level of exosomes in human serum will vary depending on many factors such as age, sex, time of sample collection (circadian rhythm/nutrition st...

Human umbilical vein endothelial cell (HUVEC) isolated from umbilical cord is widely used as endothelial cell model. However, HUVEC has been characteristically hard to maintain and showed molecular heterogeneity depending on the umbilical cord donors. Commercial HUVEC is commonly derived from European and Caucasian population which have different molecular characteristics from Asian women. This...

the operating room. Non-enzymatically isolated stromal vascular fraction contained cells expressing CD44, CD73, CD90 and CD105 that when expanded in culture differentiated along adipogenic and osteogenic lineages. When compared to enzymatic isolation, mechanical isolation methods required less time but produced lower cell yields. Two articles reported improved volume retention in fat grafts sup...

We report the isolation of Histoplasma capsulatum from a culture of the viscera of Didelphis albiventris, one of the marsupial species found in Brazil. To our knowledge, this is the first report of the isolation of this fungus from this mammalian species. This finding confirms the ubiquitous presence of H. capsulatum in nature.