Guanidine compounds have important biochemical properties. Aminoguanidine, as an example, is an anti-oxidant, a nitric oxide synthase inhibitor (NOS) which prevents nitric oxide formation, and an inhibitor of advanced glycosylation end products (AGEs). As an anti-oxidant, aminoguanidine may affect the formation of atherosclerotic lesions through protection from LDL oxidation. Inhibition of AGEs...

A simple and efficient synthesis of aryl and heteroaryl substituted pyrimidines has been developed via initial Knoevenagal, subsequent addition and final cyclization of aldehyde, ethylcyanoacetate and guanidine nitrate. Piperidine has been used as a catalyst. Short reaction time, environment friendly procedure and excellent yields are the advantages of this procedure. All synthesized compounds ...

A number of very careful studies have been made in recent years of the thermal transitions which small globular proteins undergo at low pH. These transitions reflect the destruction of the ordered conformation of the native protein, and the products of the transition have the properties of highly disordered polypeptide chains. The principal objective of such studies has, however, been to determ...

In a previous communication, it was shown that on prolonged exposure to urea at temperatures above 20” pepsin is irreversibly inactivated (1). The loss of activity is most marked in the pH range of 4.6 to 5.6 and is always accompanied by the formation of nonprotein material that originates from autodigestion of the protein. Since urea is known to affect the secondary structure of proteins by ru...

The kinetics of actin unfolding induced by guanidine hydrochloride of different concentrations was studied. The parametric representation of the kinetic dependencies of tryptophan fluorescence intensity changes recorded at two wavelengths allowed us to detect and characterize a new essentially unfolded kinetic intermediate. Its characteristics suggested that this intermediate state is a premolt...

The conventional iodine-based (I − /I 3 ) electrolyte used in dye-sensitized solar cells (DSSCs) presents several limitations, such as ∼30% absorption of visible light the wavelength range 300–500 nm and a large potential difference between Fermi level I HOMO dye. This has negative impact on characteristics DSSC transparency open circuit voltage (V oc ). In present work, series transparent elec...

Structural comparison of in vitro evolved proteins with biological proteins will help determine the extent to which biological proteins sample the structural diversity available in protein sequence space. We have previously isolated a family of nonbiological ATP binding proteins from an unconstrained random sequence library. One of these proteins was further optimized for high-affinity binding ...

Densities and viscosities of aqueous solutions of urea and guanidine hydrochloride at 250 have been measured, and equations are presented which describe their variation with concentration. Densities and viscosities of similar solutions containing additional solutes (buffers, NaCI, -mercaptoethanol) have been measured, and the possibility of predicting these results from information on solutions...

To obtain more information about the structural properties and conformational stabilities of GFP-like fluorescent proteins, we have undertaken a systematic analysis of series of green and red fluorescent proteins with different association states. The list of studied proteins includes EGFP (green monomer), zFP506 (green tetramer), mRFP1 (red monomer), "dimer2" (red dimer), and DsRed1 (red tetra...