نتایج جستجو برای: gene targeting
تعداد نتایج: 1252207 فیلتر نتایج به سال:
Yersinia pestis, a gram-negative rod belonging to the Enterobacteriaceae family, is the causative agent of plague. Classical methods of detecting the organisms are time-consuming, expensive and dangerous. The aim of the study was to design a Real-time PCR assay on the basis of the pla gene of Yersinia pestis. In this research the Real- time PCR test was optimized by using special primers for ta...
Background & Aim: Transgenic mice, of tengenerated by random integration of foreign genes into the mouse genome or by targeted mutation in a particular gene, have demonstrated to be a very effective tool for studying gene function in living things. In this review article, we discussed on the current methods of generating genetically-modified mice and their related problems and then investigated...
Fifteen non-aflatoxigenic strains of Aspergillus flavus, represent a wide range of geographic regions of Iran (six provinces include Fars, Ardebil, Guilan, Golestan, Kerman and Semnan) and vegetative compatibility groups (VCGs), were collected from corn (Zea mays L.), peanut (Arachis hypogaea L.) and pistachio (Pistachia vera L.) soils and kernels, were screened for the presence of aflatoxin bi...
Introduction: Gastric cancer is one of the most common gastrointestinal tract neoplasms. Because of its invasion, and nonspecific symptoms and signs, the disease is often diagnosed at an advanced stage with short survival. PRR11 participates in the initiation and progression of lung cancer and breast cancer by regulating important genes involved in cell cycles and tumorigenesis. In this researc...
background: tuberculous endometritis is often a diagnostic dilemma for gynecologists in the evaluation of infertility. this study evaluated and compared different diagnostic methods in tuberculous endometritis. methods: 100 infertile women were investigated for tuberculous endometritis. the endometrial samples obtained by curettage were evaluated by ziehl-neelsen (zn) staining of the smears for...
background: congenital toxoplasmosis is an important cause of spontaneous abortion worldwide. however, there is limited information on detection and genotypic characterization of t. gondii in women with recurrent spontaneous abortion (rsa). the aim of this study is molecular detection and genotypic characterization of t. gondii in formalin-fixed, paraffin-embedded fetoplacental tissues (ffpts) ...
objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. one of the effective therapeutic methods for this disease can be gene therapy by viral vectors. the capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini lcr and β-globin gene instead of lcr region. the aim of this study is to make a recombinant lentiviruses conta...
The present study investigates the applicability of nanoparticle delivery vectors for two-stage targeting that involves both cell entry by endocytosis and nuclear targeting using viral peptide signals. A nanoparticle vector consists of four components: a carrier nanoparticle, a stabilizer, targeting peptides, and a therapeutic cargo. Extensive study of bovine serum albumin (BSA)-peptide stabili...
Artificial gene alteration by homologous recombination in living cells, termed gene targeting, presents fundamental and considerable knowledge of in vivo gene function. In principle, this method can possibly be applied to any type of genes and transformable cells. However, its success is limited due to a low frequency of homologous recombination between endogenous targeted gene and exogenous tr...
We have compared the efficiency of transformation by linear DNA fragments in yeast strains carrying different numbers of homologous targets for recombination. In strains carrying dispersed copies of a target and in strains carrying tandem arrays, the frequency of transformation is proportional to the number of targets. This result is in contrast to previous studies of transformation in mammalia...
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