Background: Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A549 cell lines. Methods: Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as its...

BACKGROUND Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines. METHODS Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as i...

The desaturation-elongation of [14C]linolenic acid (n-3) in the rat small intestine was examined in homogenates of villus and crypt cells in vitro and in mesenteric lymph duct cannulated rats in vivo, using high performance liquid chromatography of fatty acid methyl esters with continuous flow measurement of 14C. Desaturation-elongation of [14C]18:3 to [14C]18:4, [14C]20:4, and [14C]20:5 (n-3) ...

We have previously determined the dietary a-linolenic requirement for membrane synthesis in the developing animal. This study measures the dietary re quirement for maintaining normal membrane compo sition in adult rats, as determined by 22:6(n-3) (docosahexaenoic acid) concentration. Sixty-day-old rats, previously fed a diet containing both linoleic and a-lino lenic acid, were divided into nine...

Inhibition of Ehrlich ascites carcinoma respiration by aqueous extracts of oxidized linoleic or linolenic acid (aqueous emulsions UV-irradiated, 90 min) was associated entirely with relatively involatile compounds which were both thiobarbituric acid (TBA)-reactive and peroxidase-reactive. Inhibitory compounds were heat stable and migrated in thin-layer chromatography with aldehydes, "hydroperox...

By employing gas chromatography, thefatty acidcontents were determinedin the root, stem,and leaf of A. pseudalhagi.The compositionof fatty acids varied among organs A.pseudalhagi. The unsaturation percentages for each part 47.78%, 48.17%,and 44.12%, respectively. dominant acid in leavesand stems was palmitic acid. contentof these organsvaried from 19.25% to 19.69%. other major γ-linolenic acidi...

Malignant gliomas are among the most devastating of cancers and are a major cause of mortality in a young population with a median survival time of 9 months following cytoreductive surgery, radiotherapy and chemotherapy. Recent studies showed that polyunsaturated fatty acids especially gamma-linolenic acid (GLA) have selective tumoricidal action especially against malignant glioma cells both in...

Twelve pumpkin cultivars (Cucurbita maxima D.), cultivated in Iowa, were studied for their seed oil content, fatty acid composition, and tocopherol content. Oil content ranged from 10.9 to 30.9%. Total unsaturated fatty acid content ranged from 73.1 to 80.5%. The predominant fatty acids present were linoleic, oleic, palmitic, and stearic. Significant differences were observed among the cultivar...