Here we describe a non-invasive method for rapid and highly reproducible genotyping of transgenic mammals with ubiquitous expression of fluorophore reporters. Hair samples from transgenic mice and pigs with systemic expression of the fluorophore reporter Venus were analyzed with a fluorescence microscope in few minutes. The hair samples can be preserved for long-term storage at ambient temperat...

We present an efficient method of introducing fluorophore labels at selected locations in a large RNA. The method is based on specific and highly efficient hybridization between a fluorophore-containing DNA oligonucleotide and a modular hairpin loop replacing a functionally unimportant hairpin loop in the RNA. We demonstrate its feasibility using a 255-nucleotide RNA derived from the catalytic ...

In this critical and timely review, the effects of anisotropic silver nanostructures on the emission intensity and photostability of a key fluorophore that is frequently used in many biological assays is examined. The silver nanostructures consist of triangular, rod-like, and fractal-like nanoparticles of silver deposited on conventional glass substrates. The close proximity to silver nanostruc...

Fluorescence spectroscopy is entrenched throughout the life sciences today and is used for a variety of applications, including the detection of chemical or biological analytes/species, in reporting molecular events or for the understanding of microenvironments, such as local viscosity, polarity and voltage measurements, to name but just a few. In the majority of these fluorescencebased measure...

A benzobisoxazole-based cruciform fluorophore forms fluorescent complexes with simple boronic acids. Through the changes of their fluorescence emission colours, these complexes can sense and qualitatively distinguish among structurally similar organic nitrogen compounds (amines and ureas) and small organic and inorganic anions. Preliminary results suggest that the intensity of this hybrid senso...

We present analytic solutions for fluorescent diffuse photon density waves originating from fluorophores distributed in thick turbid media. Solutions are derived for a homogeneous turbid medium containing a uniform distribution of fluorophores and for a system that is homogeneous except for the presence of a single spherical inhomogeneity Generally the inhomogeneity has fluorophore concentratio...