Archival formalin-fixed, paraffin-embedded and ethanol-fixed tissues represent a potentially invaluable resource for gene expression analysis, as they are the most widely available material for studies of human disease. Little data are available evaluating whether RNA obtained from fixed (archival) tissues could produce reliable and reproducible microarray expression data. Here we compare the u...

BACKGROUND Cyclosporin A (CsA) is an immunosuppressive agent which is used to prevent graft rejection and to treat autoimmune disorders. Its teratogenic effects remain unexplored despite its extensive use even during pregnancy. Current study was, therefore, undertaken to investigate the effects of CsA on the developing kidney. METHODS Twelve pregnant mice were divided into two groups, A and B...

The objective of this work was to evaluate the acute toxicity of formalin and histopathological effects on the Amazon ornamental fish, bluespotted coridora (Corydoras melanistius). A randomized design was used, with ten concentrations of formalin (40%) (0, 3, 6, 12, 25, 50, 100, 150, 200 and 250 mg.L(-1)) with four replicates and five fish per container (3L) in static system for 96 hours. The m...