We have investigated the ability of neutral and lysosomal enzymes of mouse macrophages to degrade the insoluble extracellular matrices secreted by smooth muscle cells, endothelial cells, and fibroblasts. Matrices produced by smooth muscle cells contained glycoproteins, elastin, and collagens, but matrices of endothelial cells and fibroblasts contained no elastin. Sequential enzyme digestion of ...

Macrophages, which secrete proteolytic enzymes capable of hydrolyzing elastin, collagen, and glycoproteins, may play a prominent role in the turnover of connective tissue macromolecules at inflammatory sites. Their degradative capacity has been demonstrated on purified connective tissue substrates (1-6) and on complex extracellular matrices produced by cultured smooth muscle cells (SMC), x fibr...

The repairing of injured skin tissue is a fundamental biological process essential to the continuity of life. Wound repair is a complex and dynamic process which consists of inflammation, angiogenesis, and tissue formation and remodeling [1,2]. The wound site is deposited with fibrin clots to prevent hemorrhage upon injury. Neutrophils and macrophages infiltrate the wounds and keratinocytes mig...

results migration of cells started on the fifth day. as a result of this migration, most cells were located on the surface of the scaffold and formed epiderm-like structure and some penetrated into the scaffold. by day 25, cells which had penetrated into the scaffold had various destinies such as becoming elongated spindle-shaped cells and creating blood vessel-like structures. conclusions adm ...

background: extracellular vesicles are particles ranged from 30 nm to 5µm and subcategorized into three groups; exosomes, microvesicles and apoptotic bodies, each of which have different biological impact. lack of a standard method for the detection and isolation of mvs has led to a challenging issue that is a worth considering. in this study, we isolated mvs from the conditioned medium of uc-m...

For the purpose of obtaining high bone forming efficacy, development of chitosan was attempted as a tool useful as a scaffolding device. Porous chitosan matrices, chitosan-poly(L-lactide) (PLLA) composite matrices and chitosan coated on PLLA matrices were dealt with in this research. Porous chitosan matrix was fabricated by freeze-drying and cross-linking aqueous chitosan solution. Porous chito...

The goal of our studies was to characterize the interrelationship between extracellular matrix organization and fibroblast proliferation in response to growth factors. We compared fibroblasts in monolayer culture with cells in contracted collagen matrices that were mechanically stressed or relaxed. In response to platelet-derived growth factor (PDGF), DNA synthesis by fibroblasts in mechanicall...