PURPOSE To validate tissue quality parameters for donor corneas designated for emergency grafting for corneal graft survival. METHODS In a longitudinal cohort follow-up study, 131 emergency penetrating grafts were studied. Grafts were performed with a pool of organ-cultured donor corneas designated for emergency grafting and prepared for immediate use with all safety tests performed. Assignat...

Human corneal endothelial cells have two major functions: barrier function mediated by proteins such as ZO-1 and pump function mediated by Na-K-ATPase which help to maintain visual function. However, human corneal endothelial cells are notorious for their limited proliferative capability in vivo and are therefore prone to corneal endothelial dysfunction that eventually may lead to blindness. At...

tients are at risk for lagophthalmos and exposure-related keratitis. The post-LASIK neurotrophic status of these patients may further predispose them to infectious keratitis. The first case represents, to our knowledge, the earliest postLASIK human histopathologic finding (9 days post-LASIK). The neurotrophic cornea would certainly be a risk factor in the immediate postoperative period. The fir...

A scleral contact lens lies on the sclera, not cornea, which differs from a rigid gas permeable (RGP) lens, and has shown successful clinical outcomes in patients unable to tolerate glasses or RGP lenses because of severely deformed cornea. In addition, forms tear film between cornea that stabilizes ocular surface could be useful therapeutic option. The mini-scleral is option for visual improve...

Purpose: To evaluate the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate for construction of deep anterior donor corneal lamellae. Methods: This was a prospective laboratory investigation. Twenty-five human corneal buttons were femtosecond laser cut to create thick anterior lamellae (diameter, 10mm; thickness, 500μm). The laser cuts were made using an LDV ...

Introduction: Corneal disease due to trauma, ulceration or infection has rendered more than 10 million people blind across the world. Transplantation of donor corneas is the current gold standard for the treatment of corneal disease. However, short supply and immune related complications are major concerns. Over the past two decades, corneal tissue engineering has gained significant interest as...

Our goal is to prove that during corneal perforation, when it larger than 3 mm, we should not rush perform keratoplasty (cornea transplant). First, do our best and fight the end preserve a patient's own cornea, in which amniotic membrane will help us. In 15 patients with where size of perforation exceeded 3-4 surgical treatment was performed piece applied cornea. 14 out 15, their cornea moderat...

This study aimed to evaluate the microbiota of donor rabbit corneas stored for tectonic transplantation purposes. Swabs from both corneas of 20 rabbits were carefully collected and submitted to microorganism isolation and identification. After this first swab collection, rabbits were euthanized for reasons other than this project and the eyes were enucleated. The corneas were collected and stor...

PURPOSE To study the structural features of human post-LASIK corneas. METHODS A pair of post-mortem donor corneas, from a 55-year old patient who underwent uncomplicated LASIK surgery five years previously, were bisected and fixed in 4% paraformaldehyde. The right cornea and one half of the left cornea were processed for light microscopy and scanning electron microscopy. One half of the right...