نتایج جستجو برای: dna cleavage

تعداد نتایج: 544661  

Journal: :PLoS ONE 2007
Olivier Delelis Vincent Parissi Hervé Leh Gladys Mbemba Caroline Petit Pierre Sonigo Eric Deprez Jean-François Mouscadet

BACKGROUND HIV-1 integrase (IN) catalyses the retroviral integration process, removing two nucleotides from each long terminal repeat and inserting the processed viral DNA into the target DNA. It is widely assumed that the strand transfer step has no sequence specificity. However, recently, it has been reported by several groups that integration sites display a preference for palindromic sequen...

Journal: :The Journal of biological chemistry 2001
C Buhler J H Lebbink C Bocs R Ladenstein P Forterre

A key step in the DNA transport by type II DNA topoisomerase is the formation of a double-strand break with the enzyme being covalently linked to the broken DNA ends (referred to as the cleavage complex). In the present study, we have analyzed the formation and structure of the cleavage complex catalyzed by Sufolobus shibatae DNA topoisomerase VI (topoVI), a member of the recently described typ...

Journal: :International Journal of Molecular Sciences 2021

Journal: :The Journal of biological chemistry 2008
Lyudmila Yakovleva Shengxi Chen Sidney M Hecht Stewart Shuman

Vaccinia DNA topoisomerase IB (TopIB) relaxes supercoils by forming and resealing a covalent DNA-(3'-phosphotyrosyl)-enzyme intermediate. Here we gained new insights to the TopIB mechanism through "chemical mutagenesis." Meta-substituted analogs of Tyr(274) were introduced by in vitro translation in the presence of a chemically misacylated tRNA. We report that a meta-OH reduced the rate of DNA ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2010
John M Hinz Yesenia Rodriguez Michael J Smerdon

Histones play a crucial role in the organization of DNA in the nucleus, but their presence can prevent interactions with DNA binding proteins responsible for repair of DNA damage. Uracil is an abundant mutagenic lesion recognized by uracil DNA glycosylase (UDG) in the first step of base excision repair (BER). In nucleosome core particles (NCPs), we find substantial differences in UDG-directed c...

Journal: :Journal of inorganic biochemistry 2006
Balaraman Selvakumar Venugopal Rajendiran Palanisamy Uma Maheswari Helen Stoeckli-Evans Mallayan Palaniandavar

The coordination geometry around copper(II) in [Cu(imda)(phen)(H2O)] (1) (H2imda = iminodiacetic acid, phen = 1,10-phenanthroline) is described as distorted octahedral while those in [Cu(imda)(5,6-dmp)] (2) (5,6-dmp = 5,6-dimethyl-1,10-phenanthroline) and [Cu(imda)(dpq)] (3) (dpq = dipyrido-[3,2-d:2',3'-f]-quinoxaline) as trigonal bipyramidal distorted square-based pyramidal with the imda anion...

2015
Gabriel E. Zentner Sivakanthan Kasinathan Beibei Xin Remo Rohs Steven Henikoff

Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo. Here we report the combination of ChEC with high-throughput sequencing (ChEC-seq) to map budding yeast transcription factor (TF) binding. Temporal analysis of ChEC-seq data reveals two classes of sites for TFs, one displ...

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